Journal ArticleDOI
The role of phenylacetic acid in biosynthesis of penicillin amidase in E. coli
P. S. R. Babu,Tapobrata Panda +1 more
TLDR
For improved biosynthesis of penicillin amidase by E. coli, NCIM 2400, the participation of different carboxylic acids and polyols has been studied in association with the effect of phenylacetic acid in a modified defined medium.Abstract:
For improved biosynthesis of penicillin amidase by E coli, NCIM 2400, the participation of different carboxylic acids and polyols has been studied in association with the effect of phenylacetic acid A modified defined medium has been devised for this purposeread more
Citations
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Journal ArticleDOI
Effect of mode of operation of bioreactors on the biosynthesis of penicillin amidase in Escherichia coli
TL;DR: Experimental findings show that the biosynthesis of penicillin amidase in E. coli show a little variation among batch reactor modes and significant variation on the continuous mode of operation, and analysis shows that the different reactor modes also influence periplasmic localization of the enzyme in the cell.
Journal ArticleDOI
6-aminopenicillanic acid production by intact cells of E. coli containing penicillin G acylase (PGA).
TL;DR: The recovery of 6-APA obtained by using crude intact cells of locally collected PGA producing bacterial strains as cheap biocatalyst appeared promising and the process of enzyme fermentation and 6- APA crystallization optimized during this study seems cost-effective and environment-friendly.
Journal ArticleDOI
Screening of Beta-lactam Acylase Producers from Soil and Characterization of Isolates for Substrate Specificity for Cephalosporins
TL;DR: Enzyme activity of isolate grown in media at varying pH showed it to be tolerant to alkaline pH 8.5 and the substrate specificity for various cephalosporins showed it most selective to cephalexin.
References
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Journal ArticleDOI
The isolation and kinetics of penicillin amidase from Escherichia coli
TL;DR: The enzyme was shown to be inhibited by excess substrate, benzylpenicillin, and by both of the products of hydrolysis, which was found to be competitive and by 6-aminopenicillanic acid to be non-competitive.
Journal ArticleDOI
The properties of the electrical double layer at a dropping mercury electrode in n-potassium chloride containing n-alkyltrimethylammonium ions
TL;DR: In this paper, the adsorption of n-alkyltrimethylammonium corrosion inhibitors at a dropping mercury electrode in N-potassium chloride solution was taken as far as -1.9 volts S.C.E.
Journal ArticleDOI
Penicillinamidohydrolase in Escherichia coli. III. Catabolite repression, diauxie, effect of cAMP and nature of the enzyme induction.
V Vojtísek,J Slezák +1 more
TL;DR: The differential rate of synthesis of penicillinamidohydrolase was studied in Escherichia coli growing in some chemically defined media and in a complex medium to find the highest rate of the enzyme synthesis is reached in a medium containing phenylacetic acid as the only source of carbon and energy.
Book ChapterDOI
Immobilization of Whole Microbial Cells for the Production of 6- Amino Penicillanic Acid
TL;DR: In the process of developing a most efficient immobilized cell system for the production of 6 APA, two routes have been followed: increase of the specific activity of the cell system itself and selection of the appropriate immobilization technique.
Journal ArticleDOI
Production of Escherichia coli as a source of nucleic acids
TL;DR: The case is argued for an increased use of stirred deep cultures because a greater rate of output, at a higher cell concentration and with improved control of cultural conditions can be achieved.