Journal ArticleDOI
The synthesis of sulfated dextran beads for isolation of human plasma coagulation factors II, IX, and X.
TLDR
This new chromatographic material greatly simplifies the isolation of these coagulation factors and good recoveries of proteins homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis can be obtained from human plasma in 2 days.About:
This article is published in Analytical Biochemistry.The article was published on 1980-07-01. It has received 167 citations till now. The article focuses on the topics: Sephadex & Coagulation (water treatment).read more
Citations
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Journal ArticleDOI
Kinetics of the inhibition of thrombin by hirudin.
Stuart R. Stone,Jan Hofsteenge +1 more
TL;DR: The dissociation constant for hirudin was determined by varying the concentration of hirUDin in the presence of a fixed concentration of thrombin and tripeptidyl p-nitroanilide substrate and was markedly dependent on the ionic strength of the assay.
Journal ArticleDOI
Role of the antithrombin-binding pentasaccharide in heparin acceleration of antithrombin-proteinase reactions. Resolution of the antithrombin conformational change contribution to heparin rate enhancement.
TL;DR: In this paper, the authors compared the antithrombin-binding heparin pentasaccharide and full-length heparins with respect to their interactions with antitrombin and their ability to promote inhibition and substrate reactions.
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Heparin cofactor II. Purification and properties of a heparin-dependent inhibitor of thrombin in human plasma.
TL;DR: A previously unrecognized heparin-dependent inhibitor of thrombin from human plasma is isolated and is a relatively ineffective inhibitor of coagulation factor Xa.
Journal ArticleDOI
Activation of endogenous factor V by a homocysteine-induced vascular endothelial cell activator.
TL;DR: It is indicated that increased Factor V activity associated with homocysteine-treated vascular endothelium results primarily from induction of an activator of Factor V.
Journal ArticleDOI
A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains.
Carl-Henrik Heldin,Ann Johnsson,Suzanne Wennergren,Christer Wernstedt,Christer Betsholtz,Bengt Westermark +5 more
TL;DR: A procedure for the preparation of homogeneous ODGF is described and evidence that this factor, which binds to the PDGF receptor, has a structure similar to a homodimer of PDGF A-chains is provided.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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The Purification and Mechanism of Action of Human Antithrombin-Heparin Cofactor
TL;DR: It is suggested that heparin binds to the inhibitor and causes a conformational change which results in a more favorable exposure of the arginine reactive site, allowing a rapid interaction with thrombin.
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A comparison of human prothrombin, factor IX (Christmas factor), factor X (Stuart factor), and protein S.
TL;DR: Human prothrombin, factor IX, and factor X have been idolated in high yield and characterized as the their amino-terminal sequence, molecular weight, amino acid composition, and migration in sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Journal ArticleDOI
Properties of the factor Xa binding site on human platelets.
TL;DR: An antibody that inhibits both human and bovine coagulation Factor V activity blocks both Xa binding to released platelets and the rapid thrombin formation associated with this binding, suggesting that Factor V from platelets is involved in the Xa-platelet interaction.