Journal ArticleDOI
Thiol status in human sperm.
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TLDR
The relationship between the thiol–disulfide status of human spermatozoa and routine semen analysis parameters and routine sperm parameters or fertilizing capacity in vitro was examined using a specific fluorescent probe, monobromobimane.Abstract:
The passage of spermatozoa along the epididymis is characterized by a gradual stabilization of intracellular organelles mainly through the oxidation of thiol groups. In this study, we examined the relationship between the thiol-disulfide status of human spermatozoa (using a specific fluorescent probe, monobromobimane) and routine semen analysis parameters. Fluorescence intensity was measured by spectrofluorimeter and its frequency distribution within samples, using a fluorescence-activated cell sorter. The mean proportion of reactive thiols SH/(SS + SH) in 29 semen samples was 29.8% +/- 2.5%. When comparing thiol labeling patterns, oligozoospermic samples differed from normozoospermic ones (P less than 0.05). However, within the normozoospermic group, no correlation was found between thiol-labeling patterns and routine sperm parameters or fertilizing capacity in vitro. No difference in thiol labeling patterns was found between "swim-up" and "whole semen" preparations.read more
Citations
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Relative Impact of Oxidative Stress on the Functional Competence and Genomic Integrity of Human Spermatozoa
TL;DR: Human spermatozoa exposed to increasing levels of oxidative stress achieved through the stimulation of endogenous oxidant generation with NADPH or direct exposure to hydrogen peroxide exhibited significantly elevated levels of DNA damage and yet continued to express an enhanced capacity for sperm-oocyte fusion.
Journal ArticleDOI
Protamines and male infertility.
TL;DR: The present work reviews the articles published to date on the relationship between protamines and infertility and finds evidence that altered levels of protamines may result in an increased susceptibility to injury in the spermatozoan DNA causing infertility or poor outcomes in assisted reproduction.
Journal ArticleDOI
Reactive oxygen species and sperm physiology
TL;DR: The fine balance between ROS production and scavenging, as well as the right timing and site for ROS production are of paramount importance for acquisition of fertilizing ability.
Journal ArticleDOI
Comparison of individual antioxidants of sperm and seminal plasma in fertile and infertile men
TL;DR: In seminal plasma, ascorbate, urate, sulphydryl groups, tocopherol and carotenoid concentrations were measured and it was found that within sperm, this group is the major contributor and in samples exhibiting ROS activity, asCorbate concentrations in the seminal plasma are significantly reduced.
Journal ArticleDOI
Sperm Nuclear Histone to Protamine Ratio in Fertile and Infertile Men: Evidence of Heterogeneous Subpopulations of Spermatozoa in the Ejaculate
TL;DR: It is suggested that infertile men possess a higher proportion of spermatozoa with an increased histone to protamine ratio than fertile controls.
References
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Journal ArticleDOI
Relation of mammalian sperm chromatin heterogeneity to fertility.
TL;DR: Flow cytometry of heated sperm nuclei revealed a significant decrease in resistance to in situ denaturation of spermatozoal DNA in samples from bulls, mice, and humans of low or questionable fertility when compared with others of high fertility.
Endocrine control of the development and maintenance of sperm fertilizing ability in the epididymis.
TL;DR: A tentative hypothesis is proposed that after hypophysectomy and castration the almost immediate regression of the endoplasmic reticulum inhibits the secretory process and blocks sperm maturation.
Journal ArticleDOI
The occurrence and possible functional significance of -S-S- crosslinks in sperm heads, with particular reference to eutherian mammals.
J. M. Bedford,Harold I. Calvin +1 more
TL;DR: The present light and electron microscopic study, in which the spermatozoa of many vertebrates and some invertebrates were exposed to SDS with or without dithiothreitol, demonstrates that the extreme degree of disulfide bonding in the sperm chromatin of eutherian mammals is highly unusual in a comparative sense.
Journal ArticleDOI
Transformation of sperm histone during formation and maturation of rat spermatozoa.
Y Marushige,K Marushige +1 more
TL;DR: Results suggest that phosphorylation of sperm histone is involved in the process of chromatin condensation during transformation of spermatozoa in the epididymis.