Journal ArticleDOI
Three dysregulated microRNAs in serum as novel biomarkers for gastric cancer screening
Hui Wang,Lei Wang,Zheng Wu,Rong Sun,Haifeng Jin,Jifeng Ma,Lili Liu,Rui Ling,Jun Yi,Ling Wang,Jie-fang Bian,Jianghao Chen,Nanlin Li,Shifang Yuan,Jun Yun +14 more
TLDR
Significantly elevated expression of the three miRNAs was also observed in advanced GC patients, which suggested their availability in cancer staging, and confirmed the diagnostic value of serum miR-223, mi-16, and mi-100 in GC.Abstract:
Gastric cancer (GC) is one of the most threatening diseases. The symptoms of GC are complex and hard to detect, which also contribute to the poor prognosis of GC. Besides, the current diagnosis for GC is expensive and invasive. Thus, a fast, noninvasive biomarker is urgently needed for GC screening. MicroRNAs (miRNAs) are small noncoding RNAs, which are involved in a great variety of pathological processes, particularly carcinogenesis. MiRNAs are stable in gastric juice, plasma as well as serum, which facilitate it to be a promising biomarker for cancer. In this study, we selected three novel miRNAs, i.e., miR-233, miR-16, and miR-100, to investigate their potential diagnostic value in GC screening. A total of 50 GC patients and 47 healthy controls were involved in this study. Blood serum samples were collected; RNAs were extracted and normalized with U6 snRNA as the internal control; qRT-PCR was performed for relative expression of target miRNAs. Levels of miRNAs expression were compared by Student’s t test for the comparison between two groups, and one-way ANOVA was used for multiple comparisons. The expression of miR-223, miR-16, and miR-100 was all significantly higher in GC patients than controls (all P < 0.001). All the tested miRNAs were manifested to be valuable biomarkers for GC. Relative expression of these miRNAs was significantly correlated with clinical characteristics of GC patients, such as TNM stage (P = 0.036 for miR-223; P < 0.001 for miR-100), metastatic status (P = 0.045 for miR-223; P = 0.031 for miR-16; P = 0.006 for miR-100), tumor size (P = 0.042 for miR-223; P = 0.031 for miR-16; P < 0.001 for miR-100), and differentiation grade (P = 0.036 for miR-223; P = 0.030 for miR-16; P = 0.034 for miR-100). However, in T classification, which considered both tumor size and direct extent of primary tumor, the difference in target miRNAs expression was not significant. In summary, we confirmed the diagnostic value of serum miR-223, miR-16, and miR-100 in GC. Significantly elevated expression of the three miRNAs was also observed in advanced GC patients, which suggested their availability in cancer staging.read more
Citations
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Journal ArticleDOI
New Concepts in Cancer Biomarkers: Circulating miRNAs in Liquid Biopsies
Erika Larrea,Carla Solé,Lorea Manterola,Ibai Goicoechea,María Armesto,María Arestin,María M. Caffarel,Angela M Araujo,Maria Araiz,Marta Fernandez-Mercado,Charles H. Lawrie,Charles H. Lawrie +11 more
TL;DR: One of the most promising classes of circulating cancer biomarkers: microRNAs (miRNAs) is reviewed, which means that cancer detection may be earlier and that the ability to monitor disease progression and/or treatment response represents a paradigm shift in the treatment of cancer patients.
Journal ArticleDOI
The role of miRNA and lncRNA in gastric cancer.
TL;DR: Greater attention of miRNA and lncRNA in gastric cancer can provide new insight of mechanism of cancer development and may be acted as a new anticancer target.
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Tumor-originated exosomal lncUEGC1 as a circulating biomarker for early-stage gastric cancer
Ling-Yun Lin,Li Yang,Qiang Zeng,Lin Wang,Mao-Li Chen,Ze-Hang Zhao,Guo-Dong Ye,Qi-Cong Luo,Pei-Yu Lv,Qi-Wei Guo,Bo-An Li,Cai Jianchun,Wang-Yu Cai +12 more
TL;DR: Exosomal lncUEGC1 may be promising in the development of highly sensitive, stable, and non-invasive biomarkers for EGC diagnosis because of its diagnostic accuracy, which was higher than the diagnostic accuracy of carcinoembryonic antigen.
Journal ArticleDOI
Diagnostic and prognostic potential of serum miR-7, miR-16, miR-25, miR-93, miR-182, miR-376a and miR-429 in ovarian cancer patients.
Xiaodan Meng,Simon A. Joosse,Volkmar Müller,Fabian Trillsch,Karin Milde-Langosch,Sven Mahner,Maria Geffken,Klaus Pantel,Heidi Schwarzenbach +8 more
TL;DR: The authors' data indicate the diagnostic potential ofmiR-7, miR-25, mi-R-93 and mi-429 in EOC and the prognostic potential of MiR-429, which may be promising molecules to be targeted in the treatment of EOC.
Journal ArticleDOI
Circulating microRNAs and long non-coding RNAs in gastric cancer diagnosis: An update and review.
Ya-Kai Huang,Jian-Chun Yu +1 more
TL;DR: To standardize results of global investigations of circulating miRNAs and lncRNAs biomarker studies, several recommendations for pre-analytic considerations are put forward and new insights into GC diagnosis and screening are highlighted.
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