Journal ArticleDOI
Thrombin Receptors of Human Platelets: Thrombin Binding and Antithrombin Properties of Glycoprotein I
P. Ganguly,N. L. Gould +1 more
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TLDR
Washed human platelets were solubilized and the proteins were separated by preparative gel electrophoresis in the presence of sodium dodecyl sulphate and the effect of the eluted proteins on the clotting of fibrinogen by thrombin was evaluated.Abstract:
Washed human platelets were solubilized and the proteins were separated by preparative gel electrophoresis in the presence of sodium dodecyl sulphate. The gel was cut into slices and the effect of the eluted proteins on the clotting of fibrinogen by thrombin was evaluated. The isolate from only one gel slice strongly inhibited the clotting of fibrinogen. The prolongation of the clotting time was dependent on the concentration of the protein and reached a plateau around 5 microgram. Gel electrophoresis of this isolate showed a prominent glycoprotein with an apparent Mr=150 000. Gel filtration studies with [125I]thrombin showed that the protein isolate bound a significant amount of thrombin which could be displaced with unlabelled thrombin. Another preparation from the same gel or purified gamma-globulin did not bind thrombin or prolong the clotting time of fibrinogen. Glycoprotein I was isolated from human platelets by affinity chromatography on lectin-Sepharose columns. The isolated glycoprotein prolonged the clotting of fibrinogen and bound [125I]thrombin which could be displaced by unlabelled thrombin. It is proposed that the high affinity receptor of thrombin on human platelets is glycoprotein I. In addition, the antithrombin activity of intact platelets is due to binding of thrombin to this glycoprotein.read more
Citations
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Journal ArticleDOI
Mocarhagin, a novel cobra venom metalloproteinase, cleaves the platelet von Willebrand factor receptor glycoprotein Ibalpha. Identification of the sulfated tyrosine/anionic sequence Tyr-276-Glu-282 of glycoprotein Ibalpha as a binding site for von Willebrand factor and alpha-thrombin.
TL;DR: Findings indicate that the sulfated tyrosine/anionic GP Ibalpha residues Tyr-276-Glu-282 are important for the binding of thrombin and botrocetin-dependent binding ofThrombin, but that vWF also interacts with residues within His-1-Leu-275, the consensus criteria for O-sulfation.
Journal ArticleDOI
Interactions between cancer cells and the microvasculature: a rate-regulator for metastasis.
TL;DR: The evidence shows that through a series of complex interactions with cancer cells, the microvasculature acts as a rate-regulator for the metastatic process, in addition to providing routes for cancer cell dissemination and arrest sites forcancer cell emboli.
Journal ArticleDOI
Purification and preliminary physicochemical characterization of human platelet membrane glycoprotein V.
M C Berndt,D R Phillips +1 more
TL;DR: To determine whether glycoprotein V was directly cleaved by alpha-thrombin and to permit further characterization of this glycopprotein as the potential functional thrombin receptor, glycop protein V was purified to > 98% homogeneity.
Journal ArticleDOI
The glycocalicin portion of platelet glycoprotein Ib expresses both high and moderate affinity receptor sites for thrombin. A soluble radioreceptor assay for the interaction of thrombin with platelets.
J T Harmon,G A Jamieson +1 more
TL;DR: Results demonstrate that both high and moderate affinity binding of thrombin to platelets is completely expressed in the glycocalicin portion of GPIb.
Journal ArticleDOI
Human neutrophil elastase modulates platelet function by limited proteolysis of membrane glycoproteins.
TL;DR: It is concluded that elastase inhibition of thrombin-induced platelet stimulation is accompanied by a reduction in the number ofThrombin binding sites per platelet and proteolysis of glycoprotein Ib.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
External Labeling of Cell Surface Galactose and Galactosamine in Glycolipid and Glycoprotein of Human Erythrocytes
TL;DR: Treatment of erythrocytes with galactose oxidase followed by reduction with tritiated sodium borohydride at pH 7.4 allowed the labeling of galactosyl and N-acetylgalactosaminyl residues on external surfaces of cells with tritium.
Journal ArticleDOI
The Binding of Thrombin to the Surface of Human Platelets
TL;DR: Results suggest that thrombin induces the release reaction by a complicated mechanism involving both binding and proteolytic activities, and DIP-thrombin increases the extent of [14C]serotonin release when platelets are exposed to suboptimal concentrations of nativeThrombin.
Journal ArticleDOI
Reduced thrombin binding and aggregation in Bernard-Soulier platelets.
G A Jamieson,T Okumura +1 more
TL;DR: The identical ratios of high affinity and low affinity binding sites in normals and in patients supports the idea of a single class of binding sites for thrombin on the platelet surface.
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