Journal ArticleDOI
Transformation of Medicago by Agrobacterium mediated gene transfer
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TLDR
DNA-DNA hybridization analysis showed the presence of the structural gene of the kanamycin resistant determinant in total DNA isolated from several independent transformants and clearly demonstrate the transfer, stable maintenance and functional expression of the Kanamycin resistance marker in Medicago varia cells which retain their morphogenic property.Abstract:
Shoot segments of Medicago varia genotype A2 were co-cultivated with Agrobacterium tumefaciens strain bo42 carrying pGA471, a plasmid coding for the kanamycin resistant determinant as transferable positive selection marker in plant cells (An et al., 1985). Resistant plants were regenerated at high frequency from green calli developed on inoculated stem cuttings under kanamycin selection. DNA-DNA hybridization analysis showed the presence of the structural gene of the kanamycin resistant determinant in total DNA isolated from several independent transformants. All data presented clearly demonstrate the transfer, stable maintenance and functional expression of the kanamycin resistance marker in Medicago varia cells which retain their morphogenic property.read more
Citations
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Journal ArticleDOI
Genetically Engineering Plants for Crop Improvement
TL;DR: The ability to introduce foreign genes into plant cells and tissues and to regenerate viable, fertile plants has allowed for explosive expansion of understanding of plant biology and has provided an unparalleled opportunity to modify and improve crop plants.
Journal ArticleDOI
Medicago truncatula, a model plant for studying the molecular genetics of the Rhizobium-legume symbiosis.
David G. Barker,Sylvie Bianchi,François Blondon,Yvette Dattée,Gérard Duc,Sadi Essad,Pascal Flament,Philippe Gallusci,Gérard Génier,P. Guy,Xavier Muel,J. Tourneur,Jean Dénarié,Thierry Huguet +13 more
TL;DR: By virtue of the diploid, self-fertilising and genetically homogeneous character of M. truncatula, it should be relatively straightforward to screen for recessive mutations in symbiotic genes, to carry out genetic analysis, and to construct an RFLP map for this plant.
Book ChapterDOI
Leaf disc transformation
Robert B. Horsch,Joyce E. Fry,Nancy L. Hoffmann,Jeanne Neidermeyer,Stephen G. Rogers,Robert T. Fraley +5 more
TL;DR: The Leaf disc transformation of tobacco is the paradigm for Agrobacterium-mediated transformation of plant tissues and subsequent selection and regeneration of transgenic plants and this example will be described for tobacco, using a vector that confers kanamycin resistance, pMON200.
Journal ArticleDOI
Selection of nodulation and mycorrhizal mutants in the model plant Medicago truncatula (Gaertn.) after γ-ray mutagenesis
TL;DR: Eighteen mutant lines of Medicago truncatula cv.
Journal ArticleDOI
Overexpression of the Arabidopsis H+-PPase enhanced resistance to salt and drought stress in transgenic alfalfa (Medicago sativa L.).
TL;DR: It is reported that overexpression of the AVP1 gene confers enhanced salt and drought tolerance to the transformed alfalfa and the increased potassium uptake and root activity in transgenic al falfa may be the consequences of rhizosphere acidification resulting from expression of theAVP1.
References
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Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Journal ArticleDOI
A plant DNA minipreparation: Version II
TL;DR: The topic of this report is rap,d m,croscale methods for,solat,on of plant D N A without tile use of ul tracentr ,fugatlon wEth CsCI, which is of moderately high molecular weight and serves as a satisfactory substrate for most restrlctum cndonucleases and is statable for genom,c blot analys,s.
Journal ArticleDOI
A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid
TL;DR: The interaction of two compatible plasmids, one containing the vir-region, the other carrying the T-DNA on a wide host-range replicon are reported, which allows introduction of the manipulated T- DNA into plant cells.
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