Journal ArticleDOI
Transgenic cowpea (Vigna unguiculata) seeds expressing a bean α-amylase inhibitor 1 confer resistance to storage pests, bruchid beetles
TLDR
A highly efficient Agrobacterium-mediatedcowpea transformation method for introduction of the bean (Phaseolus vulgaris) α-amylase inhibitor-1 (αAI-1) gene into a commercially important Indian cowpea cultivar, Pusa Komal and generated fertile transgenic plants.Abstract:
Cowpea is one of the important grain legumes. Storage pests, Callosobruchus maculatus and C. chinensis cause severe damage to the cowpea seeds during storage. We employ a highly efficient Agrobacterium-mediated cowpea transformation method for introduction of the bean (Phaseolus vulgaris) α-amylase inhibitor-1 (αAI-1) gene into a commercially important Indian cowpea cultivar, Pusa Komal and generated fertile transgenic plants. The use of constitutive expression of additional vir genes in resident pSB1 vector in Agrobacterium strain LBA4404, thiol compounds during cocultivation and a geneticin based selection system resulted in twofold increase in stable transformation frequency. Expression of αAI-1 gene under bean phytohemagglutinin promoter results in accumulation of αAI-1 in transgenic seeds. The transgenic protein was active as an inhibitor of porcine α-amylase in vitro. Transgenic cowpeas expressing αAI-1 strongly inhibited the development of C. maculatus and C. chinensis in insect bioassays.read more
Citations
More filters
Missing food : the case of postharvest grain losses in Sub-Saharan Africa
Nancy Morgan,Gunnar Larson +1 more
TL;DR: The postharvest losses (PHL) can and do occur all along the chain from farm to fork, which reduces real income for all consumers and especially affects the poor as mentioned in this paper.
Book ChapterDOI
Cowpea [ Vigna unguiculata (L.) Walp.]
R. Behura,Sanjeev Kumar,Bedabrata Saha,Bedabrata Saha,Manasa Kumar Panda,Mohitosh Dey,Ayan Sadhukhan,Sagarika Mishra,Shamsher Alam,Debee Prasad Sahoo,Twinkle Sugla,Lingaraj Sahoo +11 more
TL;DR: This chapter describes a detailed protocol for Agrobacterium-mediated transformation of the cowpea variety Pusa Komal, which includes explant infection to transgenic seed generation in greenhouse and putative transformed plants are selected using aminoglycoside antibiotic or mannose as sole carbon source.
Journal ArticleDOI
Achievements and Challenges in Legume Breeding for Pest and Disease Resistance
Diego Rubiales,Sara Fondevilla,Weidong Chen,Laurent Gentzbittel,Thomas J. V. Higgins,María Ángeles Castillejo,Karam B. Singh,Nicolas Rispail +7 more
TL;DR: The recent development of large scale phenotyping, genome sequencing and analysis of gene, protein and metabolite expressions can be of great help to further decipher plant-pathogen interactions and identify key resistance components that may be introgressed into crop plants through breeding.
Journal ArticleDOI
Simultaneous RGB emitting Au nanoclusters in chitosan nanoparticles for anticancer gene theranostics
TL;DR: The development of a composite consisting of highly fluorescent Au nanoclusters and the biopolymer chitosan, which could easily be converted into nanoparticles and would form a stable polyplex with suicide gene for induction of apoptosis in cervical cancer cells is reported.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Journal ArticleDOI
Assaying chimeric genes in plants: The GUS gene fusion system
TL;DR: Gene fusions can be defined its DNA constructions that result in the coding sequences from one gene (r@o,ter) being transcribed and/or translated under the direction of the controlling sequences of another gene (cmltrr).
Journal ArticleDOI
Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.
TL;DR: A large number of morphologically normal, fertile, transgenic rice plants were obtained by co-cultivation of rice tissues with Agrobacterium tumefaciens, and sequence analysis revealed that the boundaries of the T-DNA in transgenic Rice plants were essentially identical to those intransgenic dicotyledons.