Journal ArticleDOI
Transmission electron microscopy with a liquid flow cell
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TLDR
The data suggest that under appropriate conditions, TEM imaging with a liquid flow cell is a promising method for understanding the in situ behaviour of nanoscale structures in a prescribed and dynamically changing chemical environment.Abstract:
The imaging of microscopic structures at nanometre-scale spatial resolution in a liquid environment is of interest for a wide range of studies Recently, a liquid flow transmission electron microscopy (TEM) holder equipped with a microfluidic cell has been developed and shown to exhibit flow of nanoparticles through an electron transparent viewing window Here we demonstrate the application of the flow cell system for both scanning and conventional transmission electron microscopy imaging of immobilized nanoparticles with a resolution of a few nanometres in liquid water of micrometre thickness The spatial resolution of conventional TEM bright field imaging is shown to be limited by chromatic aberration due to multiple inelastic scattering in the water, and we demonstrate that the liquid in the cell can be displaced by a gas phase that forms under intense electron irradiation Our data suggest that under appropriate conditions, TEM imaging with a liquid flow cell is a promising method for understanding the in situ behaviour of nanoscale structures in a prescribed and dynamically changing chemical environmentread more
Citations
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Electron–Water Interactions and Implications for Liquid Cell Electron Microscopy
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TL;DR: In this article, the authors predict the composition of water subjected to electron irradiation in the electron microscope and reinterpret available experimental data, such as beam-induced variations in pH and colloid aggregation, in light of their predictions.
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Bubble and pattern formation in liquid induced by an electron beam.
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References
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Journal ArticleDOI
Observation of Single Colloidal Platinum Nanocrystal Growth Trajectories
Haimei Zheng,Rachel K. Smith,Young-wook Jun,Young-wook Jun,Christian Kisielowski,Ulrich Dahmen,A. Paul Alivisatos,A. Paul Alivisatos +7 more
TL;DR: Using in situ transmission electron microscopy, it is shown that platinum nanocrystals can grow either by monomer attachment from solution or by particle coalescence, and it is suggested that colloidal nanocrystal take different pathways of growth based on their size- and morphology-dependent internal energies.
Book
Scanning Electron Microscopy: Physics of Image Formation and Microanalysis
TL;DR: In this article, the authors describe the physics of a scanning electron microscope, including: Electron Optics of a Scanning Electron Microscope., Electron Scattering and Diffusion, Emission of Backscattered and Secondary Electrons, Electron Detectors and Spectrometers, Image Contrast and Signal Processing, and Electron-Beam Induced Current and Cathodoluminescence.
Journal Article
Observation of Single Colloidal Platinum Nanocrystal Growth Trajectories
TL;DR: In this article, the authors used in situ transmission electron microscopy to show that platinum nanocrystals can grow either by monomer attachment from solution onto the existing particles or by coalescence between the particles.
Journal ArticleDOI
Dynamic microscopy of nanoscale cluster growth at the solid–liquid interface
TL;DR: This work follows in real time the evolution of individual clusters, and compares their development with simulations incorporating the basic physics of electrodeposition during the early stages of growth, to analyse dynamic observations—recorded in situ using a novel transmission electron microscopy technique—of the nucleation and growth of nanoscale copper clusters during electro Deposition.
Journal ArticleDOI
Electron microscopy of whole cells in liquid with nanometer resolution
TL;DR: Liquid STEM is a unique approach for imaging single molecules in whole cells with significantly improved resolution and imaging speed over existing methods.