Transorbital Approach to the Middle Cerebral Artery of the Squirrel Monkey: A Technique for Experimental Cerebral Infarction Applicable to Ultrastructural Studies
W. R. Hudgins,Julio H. Garcia +1 more
TLDR
Modifications to a previously devised method for induction of cerebral infarct are herein described and this new surgical approach has made it possible to conduct detailed and sequential ultrastructural analysis of experimental cerebralinfarctions.Abstract:
An appropriate surgical technique for the production of cerebral infarction must fulfill, among others, the following criteria in order to be suitable for electron microscopy (EM) studies: (1) the method of arterial occlusion should yield a high percentage of infarcts with predictable average size; (2) there must be avoidance of surgical manipulation (i.e., retraction) of the cerebral tissues or exposure of the same to the atmosphere; and (3) the method for occluding the artery must be one that permits fixation by perfusion of the ischemic and nonischemic brain.
Modifications to a previously devised method for induction of cerebral infarct are herein described. This new surgical approach has made it possible to conduct detailed and sequential ultrastructural analysis of experimental cerebral infarctions.read more
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Focal cerebral ischaemia in the rat: 1. Description of technique and early neuropathological consequences following middle cerebral artery occlusion.
TL;DR: The ability to produce a consistent focal ischaemic lesion in the rodent brain provides a technical approach that is sufficiently reproducible to enable investigation of the pathophysiology of ischaemia using recently developed autoradiographic and neurochemical methods.
Journal ArticleDOI
Acute ischemic stroke: overview of major experimental rodent models, pathophysiology, and therapy of focal cerebral ischemia.
Aysan Durukan,Turgut Tatlisumak +1 more
TL;DR: Various rodent animal models, pathogenic mechanisms, and promising therapeutic approaches of ischemic stroke are focused on.
Journal ArticleDOI
Correlation between cerebral blood flow and histologic changes in a new rat model of middle cerebral artery occlusion.
H Nagasawa,Kyuya Kogure +1 more
TL;DR: A new focal ischemia model consisting of unilateral middle cerebral artery occlusion with a silicone rubber cylinder attached to a nylon surgical thread inserted through the internal carotid artery in rats that should be useful in various investigations of the influence of reperfusion on focal ischemic brain injury.
Journal ArticleDOI
Middle cerebral artery occlusion in rats: a neurological and pathological evaluation of a reproducible model.
TL;DR: The proposed new MCAO technique appears useful in reproducing large-sized infarcts of the frontoparietal cortex in rats and is able to predict the size of infarction from the neurological evaluation in 83% of the animals.
Journal ArticleDOI
Quantitative assessment of early brain damage in a rat model of focal cerebral ischaemia.
TL;DR: The volumetric assessment allows an objective assessment of drug therapy and management strategies in the treatment of cerebral infarction and can readily detect the increase in amount of structural damage in cerebral cortex following a transient episode of hypotension.
References
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Book ChapterDOI
Observations on Penetration of Serum Proteins into the Central Nervous System
TL;DR: Observations on protein penetration into the isolated chick choroid plexus indicated the presence of an active transport mechanism that may be responsible for the stromal uptake of fluorescein labeled albumin.
Journal Article
Effect of low molecular weight dextran on infarction after experimental occlusion of the middle cerebral artery.
Journal ArticleDOI
Experimental cerebrovascular occlusion in dog.
TL;DR: In dog and monkey permanent occlusion of the middle cerebral artery ordinarily produces a lateralized neurological deficit, the degree of impairment varying from one animal to the next, and changes in the brain's electrical activity during and for short intervals after an experimental occlusions are also variable.
Journal ArticleDOI
The Effect of Electrocautery, Atmospheric Exposure, and Surgica Retraction on the Permeability of the Blood-Brain-Barrier
W. R. Hudgins,Julio H. Garcia +1 more
TL;DR: Alterations in the permeability to Evans-blue dye of the blood-brain-barrier were encountered in a group of squirrel monkeys that were being readied for surgical occlusion of the middle cerebral artery.
Journal ArticleDOI
Ultrastructure of the neurons of the cerebral cortex.
F.R. Freemon,George H. Collins +1 more
TL;DR: Rat cerebral cortex prepared by osmium tetroxide perfusion was studied with the electron microscope and it is proposed that increased protein synthesis by the pyramidal neuron with increased rate of transfer of RNA from nucleus to cytoplasm is responsible for differences.