Trehalose synthesis genes are controlled by the putative sigma factor encoded by rpoS and are involved in stationary-phase thermotolerance in Escherichia coli.
TLDR
It is shown that the genes otsA, otsB, treA, and osmB, previously known to be osmotically regulated, are also induced during transition into stationary phase in a sigma S-dependent manner.Abstract:
The rpoS (katF) gene of Escherichia coli encodes a putative sigma factor (sigma S) required for the expression of a variety of stationary phase-induced genes, for the development of stationary-phase stress resistance, and for long-term starvation survival (R. Lange and R. Hengge-Aronis, Mol. Microbiol. 5:49-59, 1991). Here we show that the genes otsA, otsB, treA, and osmB, previously known to be osmotically regulated, are also induced during transition into stationary phase in a sigma S-dependent manner. otsA and otsB, which encode trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase, respectively, are involved in sigma S-dependent stationary-phase thermotolerance. Neither sigma S nor trehalose, however, is required for the development of adaptive thermotolerance in growing cells, which might be controlled by sigma E.read more
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Desiccation tolerance of prokaryotes.
TL;DR: The present review considers a number of the features that appear to be critical to the withstanding of a long-term water deficit, including the elaboration of a conspicuous extracellular glycan, synthesis of abundant UV-absorbing pigments, and maintenance of protein stability and structural integrity.
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Oxidative stress responses in Escherichia coli and Salmonella typhimurium.
TL;DR: The products of Oxy-R- and SoxRS-regulated genes, such as catalases and superoxide dismutases, are involved in the prevention of oxidative damage, whereas others play a role in the repair of oxidativeDamage.
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The stationary phase of the bacterial life cycle
TL;DR: This chapter reviews the recent advances made in understanding of the molecular events that allow some gram-negative bacteria to survive prolonged periods of starvation and presents three aspects of stationary phase: entry into stationary phase, responses during prolonged starvation, and reentry into the growth cycle.
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The RpoS-mediated general stress response in Escherichia coli.
TL;DR: The complex transition from exponential growth to stationary phase has been partially dissected by analyzing the induction of RpoS after specific stress treatments, and a better understanding has been led to a better, but still far from complete, understanding of how stresses lead to RPOS induction and what RPoS-dependent genes help the cell deal with the stress.
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A novel DNA-binding protein with regulatory and protective roles in starved Escherichia coli.
TL;DR: The existence of similar proteins, heretofore with no known function, in bacterial species distantly related to Escherichia coli suggests that Dps may define a novel class of widely conserved DNA-binding proteins.
References
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Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
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Preservation of Membranes in Anhydrobiotic Organisms: The Role of Trehalose
TL;DR: Results obtained with infrared spectroscopy indicate that trehalose and DPPC interact by hydrogen bonding between the OH groups in the carbohydrate and the polar head groups of DPPC, and this interaction is specific totrehalose.
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Identification of a central regulator of stationary‐phase gene expression in Escherichia coli
TL;DR: It is suggested that this novel sigma subunit of RNA polymerase defined by csi 2/katF/appR is a central early regulator of a large starvation/stationary phase regulon in E. coli and proposed ‘rpoS’ (‘σs’) as appropriate designations.
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Increased thermal stability of proteins in the presence of sugars and polyols
TL;DR: The difference between the partial molar volume of the sugar or polyol and its van der Waals volume was used as a rough quantitative measure of the structure-making or structure-breaking effect.