Journal ArticleDOI
Triplet excited states of polycyclic aromatic compounds as probes of their microenvironment in serum albumin complexes.
TLDR
Using flash photolysis techniques, the triplet excited states of benzo(a)pyrene, pyrene, benz( a)anthracene and other aromatic hydrocarbons have been detected in complexes of bovine (and human) serum albumin dissolved in aqueous solutions at room temperature.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1975-06-16. It has received 9 citations till now. The article focuses on the topics: Bovine serum albumin & Pyrene.read more
Citations
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Journal ArticleDOI
Fluorescence quenching studies with proteins.
TL;DR: A review of the use of the technique of solute fluorescence quenching to study the structure and dynamics of proteins and a number of factors are discussed that must be considered in analyzing such data.
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A spectroscopic technique for measuring slow rotational diffusion of macromolecules. 1: Preparation and properties of a triplet probe.
TL;DR: Triplet state lifetimes of air-equilibrated solutions of eosin-protein conjugates are in the order of 10 mus, demonstrating that the probe is protected from oxygen quenching by the protein.
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Excited triplet states used to study biological macromolecules at room temperature
TL;DR: The appearance and disappearance of the triplet state molecule and the influence of diffusion on quenching are summarized.
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A Long-Lived Triplet State Is the Entrance Gateway to Oxidative Photochemistry in Green Fluorescent Proteins.
TL;DR: It is suggested that it is this triplet state that lies at the origin of oxidative photochemistry in green FPs, leading to phenomena such as so-called "oxidative redding", "primed photoconversion", or, in a manner similar to that previously observed for organic dyes, redox induced blinking control with the reducing and oxidizing system.
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The triplet state as a probe of dynamics and structure in biological macromolecules.
TL;DR: The characteristics of triplet excited states, with special reference to tryptophan in proteins, and aromatic molecules which have been employed as probes in studying the dynamics and other properties of proteins and DNA are discussed.
References
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Journal ArticleDOI
Quenching of fluorescence by oxygen. A probe for structural fluctuations in macromolecules.
TL;DR: In this article, the fluorescence of various fluorophores by molecular oxygen has been studied in aqueous and nonaqueous solutions equilibrated with oxygen pressures up to 100 atm.
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Oxygen quenching of pyrenebutyric acid fluorescence in water. A dynamic probe of the microenvironment.
Vaughan Wm,Gregorio Weber +1 more
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The Reversible Expansion of Bovine Serum Albumin in Acid Solutions1
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Oxygen quenching of aromatic triplet states in solution. Part 1
TL;DR: In this paper, the rate constants for oxygen quenching of aromatic hydrocarbon triplets have been measured by the laser flash photolysis technique, and the importance of restrictive Franck-Condon factors which are determined by the hydrocarbon is discussed.
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Dye‐sensitized photooxidation of proteins*
TL;DR: The present paper is concerned with the protein aspects of photodynamic effects on proteins, and not all cases of the dye-sensitized photooxidation of proteins can be interpreted in terms of a singlet-oxygen mechanism.