What is the Sugar Code
Hans-Joachim Gabius,Mare Cudic,Tammo Diercks,Herbert Kaltner,Jürgen Kopitz,Kevin H. Mayo,Paul V. Murphy,Stefan Oscarson,René Roy,Andreas Schedlbauer,Stefan Toegel,Antonio A. Romero +11 more
TLDR
In this article, a large panel of sugar receptors (lectins) has developed based on more than a dozen fold changes in the glycan-lectin recognition process, and a large number of post-binding events.Abstract:
A code is defined by the nature of the symbols, which are used to generate information-storing combinations (e. g. oligo- and polymers). Like nucleic acids and proteins, oligo- and polysaccharides are ubiquitous, and they are a biochemical platform for establishing molecular messages. Of note, the letters of the sugar code system (third alphabet of life) excel in coding capacity by making an unsurpassed versatility for isomer (code word) formation possible by variability in anomery and linkage position of the glycosidic bond, ring size and branching. The enzymatic machinery for glycan biosynthesis (writers) realizes this enormous potential for building a large vocabulary. It includes possibilities for dynamic editing/erasing as known from nucleic acids and proteins. Matching the glycome diversity, a large panel of sugar receptors (lectins) has developed based on more than a dozen folds. Lectins 'read' the glycan-encoded information. Hydrogen/coordination bonding and ionic pairing together with stacking and C-H/π-interactions as well as modes of spatial glycan presentation underlie the selectivity and specificity of glycan-lectin recognition. Modular design of lectins together with glycan display and the nature of the cognate glycoconjugate account for the large number of post-binding events. They give an entry to the glycan vocabulary its functional, often context-dependent meaning(s), hereby building the dictionary of the sugar code.read more
Citations
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Complete 1H and 13C NMR chemical shift assignments of mono-to tetrasaccharides as basis for NMR chemical shift predictions of oligo- and polysaccharides using the computer program CASPER.
Axel Furevi,Alessandro Ruda,Thibault Angles d’Ortoli,Hani Mobarak,Jonas Ståhle,Christoffer Hamark,Carolina Fontana,Olof Engström,Patricia Apostolica,Göran Widmalm +9 more
TL;DR: In this paper , the authors used NMR spectroscopy to assign 1H and 13C NMR chemical shifts of 30 monosaccharides, 17 disaccharide, 10 trisacide, and one tetrasacide.
Posted ContentDOI
A pore-forming β-trefoil lectin with specificity for the tumor-related glycosphingolipid Gb3
Simona Notová,François Bonnardel,Francesca Rosato,Lina Šiukštaitė,Jessica Schwaiger,Nicolai V. Bovin,Annabelle Varrot,W. Roemer,Frédérique Lisacek,Anne Imberty +9 more
TL;DR: SaroL-1 from the lower eukaryote Salpingoeca rosetta was predicted to contain both β-trefoil and aerolysin-like pore-forming domain, with a stronger affinity for cancer-related α-galactosylated epitopes such as glycosphingolipid Gb3 embedded in giant unilamellar vesicles or cell membranes.
Journal ArticleDOI
Combination of High-Resolution Multistage Ion Mobility and Tandem MS with High Energy of Activation to Resolve the Structure of Complex Chemoenzymatically Synthesized Glycans.
David Ropartz,Mathieu Fanuel,Simon Ollivier,Adrien Lissarrague,Mounir Benkoulouche,Laurence A. Mulard,Isabelle André,David Guieysse,Hélène Rogniaux +8 more
TL;DR: The use of complementary strategies based on mass spectrometry to evaluate the ability of 14 engineered mutants of sucrose-utilizing α-transglucosylases to produce type/group-specific Shigella flexneri pentasaccharide bricks from a single lightly protected non-natural tetrasaccharides acceptor substrate illustrates the potential of the recently described powerful IMS and CTD-MS methods for the precise structural characterization of complex glycans.
Journal ArticleDOI
The choanoflagellate pore-forming lectin SaroL-1 punches holes in cancer cells by targeting the tumor-related glycosphingolipid Gb3
Simona Notová,François Bonnardel ,Francesca Rosato,Lina Šiukštaitė,Jessica Schwaiger,Jia-Hui Lim,Nicolai V. Bovin,Annabelle Varrot,Yukio Ogawa,Winfried Römer,Frédérique Lisacek,Anne Imberty +11 more
TL;DR: In this paper , a database of 44714 β-trefoil candidate lectins across 4497 species was searched for original domain combinations, which led to single out SaroL-1 in the choanoflagellate Salpingoeca rosetta.
Journal ArticleDOI
Multicomponent reaction derived small di- and tri-carbohydrate-based glycomimetics as tools for probing lectin specificity
TL;DR: A novel set of glycomimetic ligands through protection/deprotection multicomponent reactions (MCR) approach demonstrated different binding abilities for plant lectins Con A and UEA I, and human galectin-3.
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