Whole-body arginine dimethylation is associated with all-cause mortality in adult renal transplant recipients.
TLDR
In this paper, the authors measured the excretion rate of DMA in 24-h collected urine samples of the RTR and of healthy kidney donors in the cohort, with the aim of quantitate whole-body asymmetric (ADMA, DMA) and symmetric (SDMA) arginine-dimethylation.Abstract:
Arginine residues in proteins can be singly or doubly methylated post-translationally. Proteolysis of arginine-methylated proteins provides monomethyl arginine, asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). ADMA and SDMA are considered cardiovascular risk factors, with the underlying mechanisms being not yet fully understood. SDMA lacks appreciable metabolism and is almost completely eliminated by the kidney, whereas ADMA is extensively metabolized to dimethylamine (DMA), with a minor ADMA fraction of about 10% being excreted unchanged in the urine. Urinary DMA and ADMA are useful measures of whole-body asymmetric arginine-dimethylation, while urinary SDMA serves as a whole-body measure of symmetric arginine-dimethylation. In renal transplant recipients (RTR), we previously found that higher plasma ADMA concentrations and lower urinary ADMA and SDMA concentrations were associated with a higher risk of all-cause mortality. Yet, in this RTR collective, no data were available for urinary DMA. For the present study, we additionally measured the excretion rate of DMA in 24-h collected urine samples of the RTR and of healthy kidney donors in the cohort, with the aim to quantitate whole-body asymmetric (ADMA, DMA) and symmetric (SDMA) arginine-dimethylation. We found that lower DMA excretion rates were associated with higher all-cause mortality, yet not with cardiovascular mortality. In the healthy donors, kidney donation was associated with considerable decreases in ADMA (by - 39%, P < 0.0001) and SDMA (by - 21%, P < 0.0001) excretion rates, yet there was no significant change in DMA (by - 9%, P = 0.226) excretion rate. Our results suggest that protein-arginine dimethylation is altered in RTR compared to healthy kidney donors and that it is pronouncedly shifted from symmetric to asymmetric arginine-dimethylation, with whole-body protein-arginine dimethylation being almost unaffected.read more
Citations
More filters
Journal ArticleDOI
Post-translational modifications (PTM): analytical approaches, signaling, physiology and pathophysiology-part I
Journal ArticleDOI
Urinary excretion of amino acids and their advanced glycation end-products (AGEs) in adult kidney transplant recipients with emphasis on lysine: furosine excretion is associated with cardiovascular and all-cause mortality.
Svetlana Baskal,Adrian Post,Daan Kremer,Alexander Bollenbach,Stephan J. L. Bakker,Dimitrios Tsikas +5 more
TL;DR: In this article, the authors measured 24-hour urinary excretion of Lys, CML, and furosine in stable kidney transplant recipients and 41 healthy kidney donors before and after donation.
Journal ArticleDOI
Love is in the hair: arginine methylation of human hair proteins as novel cardiovascular biomarkers
Alistair James Marsden,David R. J. Riley,Stefan T. Birkett,Stefan T. Birkett,Quentin Rodriguez-Barucg,Barbara-Ann Guinn,Sean Carroll,Lee Ingle,Thozhukat Sathyapalan,Pedro Beltran-Alvarez +9 more
TL;DR: This article showed that human hair proteins are post-translationally modified by arginine methylation (ArgMe) using western blot, proteomic data mining and mass spectrometry.
Journal ArticleDOI
Arginine-hydrolyzing enzymes for electrochemical biosensors
TL;DR: In this article , the peculiarities of electrochemical biosensors for Arg assay based on the use of Arg-degrading enzymes and on the analysis of their advantages as compared to other approaches.
Journal ArticleDOI
Nutritional Predictors of Cardiovascular Risk in Patients after Kidney Transplantation-Pilot Study
S. Czaja-Stolc,Paulina Wołoszyk,Sylwia Małgorzewicz,Andrzej Chamienia,Michał Chmielewski,Zbigniew Heleniak,Alicja Dębska-Ślizień +6 more
TL;DR: Even in stable KTRs a relationship between inflammatory state, nutritional status, graft function and endothelial dysfunction biomarkers was observed and diabetes mellitus (DM) was associated with higher levels of ADMA and FGF-23.
References
More filters
Journal ArticleDOI
Histone methylation: a dynamic mark in health, disease and inheritance
Eric L. Greer,Yang Shi +1 more
TL;DR: This work provides a broad overview of how histone methylation is regulated and leads to biological outcomes and suggests its links to disease and ageing and possibly to transmission of traits across generations are illustrated.
Journal ArticleDOI
The Role of Nitric Oxide on Endothelial Function
Dimitris Tousoulis,Anna-Maria Kampoli,Costas Tentolouris,Nikolaos Papageorgiou,Christodoulos Stefanadis +4 more
TL;DR: The important role of nitric oxide in physiological endothelium is discussed and the significance of this molecule in pathological states altering the endothelial function is pinpointed.
Journal ArticleDOI
Arginine Methylation: The Coming of Age
TL;DR: A review of the recent molecular advances that have been uncovered in normal and diseased mammalian cells linking protein arginine methyltransferases to diseases such as cancer and metabolic, neurodegenerative, and muscular disorders is described.
Journal ArticleDOI
Asymmetric Dimethylarginine Causes Hypertension and Cardiac Dysfunction in Humans and Is Actively Metabolized by Dimethylarginine Dimethylaminohydrolase
Vinod Achan,Michael Broadhead,Mohammed Malaki,Guy St. J. Whitley,James Leiper,R. J. Macallister,Patrick Vallance +6 more
TL;DR: The cardiovascular effects of a systemic increase in ADMA in humans are defined, similar to changes seen in diseases associated with ADMA accumulation, and data indicate that ADMA is metabolized by DDAHs extensively in humans in vivo.
Related Papers (5)
Urinary Dimethylamine (DMA) and Its Precursor Asymmetric Dimethylarginine (ADMA) in Clinical Medicine, in the Context of Nitric Oxide (NO) and Beyond.
ADMA: a novel risk factor that explains excess cardiovascular event rate in patients with end-stage renal disease.
Rainer H. Böger,Carmine Zoccali +1 more