Showing papers on "Acacetin published in 2008"

Acacetin, a natural flavone, selectively inhibits human atrial repolarization potassium currents and prevents atrial fibrillation in dogs

Abstract: Background— The development of atrium-selective antiarrhythmic agents is a current strategy for inhibiting atrial fibrillation (AF). The present study investigated whether the natural flavone acacetin from the traditional Chinese medicine Xuelianhua would be an atrium-selective anti-AF agent. Methods and Results— The effects of acacetin on human atrial ultrarapid delayed rectifier K+ current (IKur) and other cardiac ionic currents were studied with a whole-cell patch technique. Acacetin suppressed IKur and the transient outward K+ current (IC50 3.2 and 9.2 μmol/L, respectively) and prolonged action potential duration in human atrial myocytes. The compound blocked the acetylcholine-activated K+ current; however, it had no effect on the Na+ current, L-type Ca2+ current, or inward-rectifier K+ current in guinea pig cardiac myocytes. Although acacetin caused a weak reduction in the hERG and hKCNQ1/hKCNE1 channels stably expressed in HEK 293 cells, it did not prolong the corrected QT interval in rabbit hearts....

Analysis of flavonoids in propolis and Ginkgo biloba by micellar electrokinetic capillary chromatography.

Abstract: A micellar electrokinetic capillary chromatography (MEKC) method has been developed for simultaneous determination of 10 bioactive flavonoids: rutin, apigenin, luteolin, eriodictyol, kaempferol, chrysin, acacetin, flavanone, flavone, and fisetin. The effect of several parameters, such as UV detection wavelength, buffer pH, buffer concentration, sodium dodecyl sulfate (SDS) concentration, β-cyclodextrin (β-CD) concentration, separation voltage, and injection time on the separation of these flavonoids were systematically investigated. The 10 flavonoids were successfully separated within 18 min in 20 mM Na2B4O7−10 mM NaH2PO4 buffer (pH 9.7) containing 100 mM SDS and 16 mM β-CD at a separation voltage of 19 kV, with UV detection at 254 nm. Regression analysis revealed a good linear relationship between the peak area of each analyte and its concentration with detection limits (S/N = 3), ranging from 0.15 to 1.36 μg mL−1. This method could simultaneously quantify the 10 flavonoids and be used in the quality con...

Studies on chemical constituents in Huangjuhua (flowers of Chrysanthemum morifolium)

Abstract: Objective To study the chemical constituents in Huangjuhua (flowers of Chrysanthemum morifolium). Method The chemical constituents were isolated by various column chromatographic methods and structurally elucidated by NMR and MS evidences. Result Eleven compounds were obtained and identified as luteolin (1), quercetin (2), acacetin 7-O-beta-D-(3"-acetyl) -glucopyranoside (3), luteolin 7-O-beta-D-(6"-acetyl)-glucopyranoside (4), hesperetin 7-O-beta-D-glucopyranoside (5), acacetin 7-O-beta-D-glucopyranoside (6), diosmetin 7-O-beta-D-glucopyranoside (7), apigenin 7-O-beta-D-glucopyranoside (8), hesperidin (9), linarin (10), and luteolin 7-O-beta-D-glucopyranoside (11). Conclusion Compounds 4, 5 were isolated from the plant for the first time.

Flavonoids, anti-inflammatory activity and cytotoxicity of Macfadyena unguis-cati L

Abstract: Ethyl acetate and ethanol extracts of unflowering aerial part of Macfadyena unguis-cati L.(Fam. Bignoniaceae) were found to be rich in phenolic compounds. From ethyl acetate extract,six flavonoids were identified, 8, methoxy, acacetin, 7-O glucoside; 6, methoxy apigenin 7-O glucoside; 4`-O methyl scutellarin, 6- O apiosyl galactoside; acacetin, 7-O glucosyl, 8-C rhamnosyl, 3-O-α arabinofuranoside; 4`-methyl, 6- methoxy kaempferol, 7-O, 8-C diglucoside and vicenin II were isolated, while 6, methoxy, acacetin 7-O glucoside; and quercitrin were isolated from ethanol extract. These compounds were characterized and identified by their physicochemical and spectral data. The crude ethanol extract exhibited significant anti-inflammatory activity (80.47%) and moderate cytotoxic activity against lung cell line. Keywords : Macfadyena unguis-cati L., Bignoniaceae, flavonoids, anti-inflammatory, cytotoxicity. African Journal of Traditional and Complementary Medicine Vol. 5 (1) 2008: pp. 18-26

Antifeedant activities of secondary metabolites from Ajuga nipponensis against Plutella xylostella

Abstract: Antifeedant activities of seven chemicals isolated from Ajuga nipponensis were examined in a bioassay against 3 rd instar larvae of Plutella xylostella. Five compounds (luteolin, stigmasterol, acacetin, 20-hydroxyecdysone and fraction 1) showed significant antifeedant activities, with the AFI50 values of 1935.02, 2515.94, 6589.58, 287.58, 2171.31µg/ml after 24h and 1853.20, 3812.24, 2581.43, 103.42, 1429.75µg/ml respectively after 48h of treatment. The synergistic effects of mixtures of secondary compounds were studied and antifeedant index was directly related to the component and the proportion of the mixture i.e., no synergism existed between luteolin and 20hydroxyecdysone mixture, but the values of CTC for fraction 1, stigmasterol, acacetin, luteolin and 20-hydroxyecdysone mixture were up to 627.07 and 111.72 after 24 and 48h, respectively. In addition, only apigenin showed effectiveness against Diamondback moth, with a high mortality percentage and low consumed leaf area when mixed with avermectins.

Studies on chemical constituents from flowers of Chrysanthemum indicum

Abstract: Objective To study the chemical constituents of the flowers of Chrysanthemum indicum. Methods Chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography. The structures were identified by means of physico-chemical and spectral data. Results From the 80% ethanol extract of the material, seven compounds were isolated. Their structures were identified as acacetin(1), acacetin-7-O-(6"-O-acetyl) beta-D-glucopyranoside (2), linarin (3), apigenin-7-O-beta-D-glucopyranoside(4), chlorogenic acid (5), vanillic acid(6) and sucrose(7), respectively. Conclusion Compounds 2, 4, 6 and 7 are isolated from C. indicum for the first time.

A new flavonol derivative from Fagopyrum dibotrys

Abstract: A new flavonol derivative 3, 8-dihydroxy-10-methoxy-5-H-isochromeno[4, 3-b]chromen-7-one (1) together with four known compounds, glutinone (2), luteolin (3), acacetin 7-O-α-L-rhamnopyranosyl- (1→6)-β-D-glucopyranoside (4), and rutin (5) were isolated from the dried roots of Fagopyrum dibotrys. Their structures were determined by UV, IR, MS, 1H, and 13C NMR spectroscopic analysis, including 2D NMR.

Study on flavonoiels and phenolic acids from the herb of Lygodium japonicum

Abstract: OBJECTIVE To study the chemical constituents of the herb of Lygodium japonicum. METHODS The constituents isolated by column chromatography and their structrues were elucidated through spectroscopic analysis. RESULTS The compounds were identified as 3,4-dihydroxyl benzoic acid 4-O-beta-D-(4'-O-methyl) glucopyranoside (I), protocatechuic acid (II), acacetin 7-O-(6'-O-a-L-rhamnopyranosyl)-beta-sophoroside (III), 6,8-di-glucosylapigenin (vinenin) (IV), tricin-7-O-beta-D-glucopyranoside (V), 2-anilino-1,4-naphthoquinone (VI). CONCLUSION All these compounds are obtained from Lygodiaeae for the first time.

Use of Flavone Compounds as Potassium Channel Inhibitors

Abstract: This invention provides a method for treating or preventing human atrial arrhythmia (fibrillation) using the leading flavone compound acacetin, and its derivatives and analogues that inhibit the ultra-rapidly-activating delayed rectifier potassium current (I Kur or I Ksus ), transient outward potassium (I to ), and acetylcholine-activated potassium current (I K.ACh ).

[Water-soluble constituents from roots of Capparis tenera].

Abstract: Objective To study the chemical constituents of the roots of Capparis tenera. Method The chemical constituents were isolated and repeatedly purified by kinds of column chromatography and the structures were elucidated by the NMR spectra and physicochemical properties. Result Eight compounds were isolated and identified as erigeside C (1), glucosuringic acid (2), vanillic acid 4-O-beta-D-glucoside (3-methoxy 4-glucosyl-benzoic acid) (3), 4-O-beta-D-glucopyranosylbenzoate (4), 3', 5'-dimethoxy- 4-O-beta-D-glucopyranosyl-cinnamic acid (5), tachioside (6), 2, 3-dihydroxy-1-(4-hydroxyl-3, 5-dimethoxyphenyl)-1-propanone (7) and acacetin 7-rutinoside (8). Conclusion Compounds 1-8 were all isolated from this plant for the first time and the compound 8 was isolated from this gene for the first time.

Cosmetic composition containing tilianin or acacetin

Abstract: A cosmetic composition comprising tilianin or acacetin is provided to reduce the bio-synthesis of MMP-1 caused by UV and promote the bio-synthesis of type I pro-collagen, thereby delaying skin aging and inhibiting wrinkle formation. A cosmetic composition for anti-aging or anti-wrinkle comprises at least one selected from the group consisting of tilianin represented by a formula(1) and acacetin represented by a formula(2), wherein the acacetin is prepared by acid-hydrolysis, alkali hydrolysis or enzyme hydrolysis of an extract of Teucrium veronicoides or the tilianin. Further, the cosmetic composition is in a form of cosmetic water, cream, essence, eye cream, cleansing cream, cleansing form, cleansing water and pack.

Phytochemical and Antimicrobial Studies on the Seeds of Mucuna Monosperma DC

Abstract: The phytochemical study of the seeds of Mucuna monosperma DC. revealed the presence of flavonoids, sterols, triterpenoids. The separated compounds were identified based on their melting points, IR, 1H NMR and mass spectral data, such compounds were acacetin, luteolin, b-sitosterol, stigmasterol, ursolic acid and betulinic acid. The antimicrobial activity of chloroform and methanolic extract of seed powder was investigated. The methanolic extract exhibited marked antibacterial activity against gram positive, gram negative organisms and fungi.