Showing papers on "Stigmasterol published in 2001"
TL;DR: The phytosterol compositions in unsaponifiables of fig (Ficus carica, var. Mission) fruit and 3 structural components of the branches; and the fatty acid composition of fig fruits were studied using gas chromatography/mass spectrometry.
Abstract: The phytosterol compositions in unsaponifiables of fig (Ficus carica, var. Mission) fruit and 3 structural components of the branches; and the fatty acid composition of fig fruits were studied using gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The phytosterols were determined from the trimethylsilyl ether (TMS) derivatives of the unsaponifiable samples. Fourteen compounds were separated from fig fruit; 13, 10, and 6 in bark, stem, and pith, respectively. Sitosterol was the most predominant sterol in all parts. Also detected were campesterol, stigmasterol, and fucosterol. Fatty acids in fig fruit, determined as their methyl esters, were myristic (14:0), palmitic (16:0), stearic (18:0), oleic (18:1), linoleic (18:2), and linolenic (18:3) acids.
122 citations
TL;DR: A narrow-bore HPLC-UV method was developed for the analysis of two of the more abundant naturally occurring phytosterols in vegetable oils: sitosterol and stigmasterol and good accuracy was statistically demonstrated since no matrix effect was found for both the analytes.
104 citations
TL;DR: In this paper, the antioxidative properties of various solvent extracts of Hsian-tsao (Mesona procumbens Hemsl) were investigated, and the results showed that for the same concentration of 0.2 g/kg, the antioxidant activity of various Hsiansa extracts, especially the ethyl acetate extract, exhibited 93% inhibition of linoleic acid, were greater than those of BHA and αtocopherol.
Abstract: The antioxidative activities of various solvent extracts of Hsian-tsao (Mesona procumbens Hemsl.) were investigated. The results showed that for the same concentration of 0.2 g/kg, the antioxidative activities of various Hsian-tsao extracts, especially the ethyl acetate extract, which exhibited 93% inhibition of peroxidation of linoleic acid, were greater than those of BHA and α -tocopherol. When the ethyl acetate extract of Hsian-tsao (EEHT) was separated into nine fractions by silica gel chromatography, fraction II showed high yield and high antioxidative activity. Fraction II was further separated into five subfractions using silica gel chromatography. Subfraction IIC was then isolated by preparative HPLC equipped with a silica column and four components were identified as stigmasterol, β -sitosterol, oleanolic acid, and ursolic acid by UV, EI-MS,1H-, and13SC-NMR. The descending order of antioxidative activity for those compounds was: EEHT>BHA>α -tocopherol>ursolic acid>oleanolic acid>β -sitosterol>stigmasterol.
63 citations
TL;DR: In this paper, a study of Mikania stipulacea yielded lupeol, a-amyrin, stigmasterol, b-sitosterol, Campesterol, campesterol and b-stigmasteryl glucopyranoside, the coumarin isoscopoletin, and vanillic, cinnamoyl grandifloric and ent-kaurenoic acids.
Abstract: Phytochemical study of Mikania stipulacea yielded lupeol, a-amyrin, stigmasterol, b-sitosterol, campesterol, b-sitosteryl glucopyranoside, stigmasteryl glucopyranoside, the coumarin isoscopoletin, and vanillic, cinnamoylgrandifloric and ent-kaurenoic acids, besides two new diterpene acids: ent-9a-hydroxy-15b-E-cinnamoyloxy-16-kauren-19-oic and ent-9a-hydroxy-15b-Z-cinnamoyloxy-16-kauren-19-oic. IR, 1H and 13C NMR and MS spectroscopic analyses were used for the identification of these compounds.
30 citations
TL;DR: Phytochemical investigation of the flowers of Wedelia paludosa afforded two diterpenes and three steroids, stigmasterol, 3b-O-b-D-glycopyranosyl sitosterol and 3b
Abstract: Phytochemical investigation of the flowers of Wedelia paludosa afforded two diterpenes, ent-kaur-16-en-19-oic acid and ent-kaur-9(11),16-dien-19-oic acid, three acylated triterpenes, 3b-O-hexa-decanoylolean-12-en-28-oic acid, 3b-O-pentadecanoylolean-12-en-28-oic acid and 3b-O-tetradecanoylolean-12-en-28-oic acid, and three steroids, stigmasterol, 3b-O-b-D-glycopyranosyl sitosterol and 3b-O-b-D-glycopyrano-sylstigmasterol. The substances were identified by their spectral data.
24 citations
TL;DR: In this article, the gas phase pyrolysis of stigmasterol was investigated using molecular beam mass spectrometry to study the reaction pathways that lead to the formation of aromatic compounds and to build an empirical kinetic model.
21 citations
TL;DR: The content and composition of free sterols in a number of Astragalus species from Iran were presented and beta-Sitosterol was the main free sterol in both roots and seeds.
20 citations
TL;DR: In this paper, the synthesis of a side-chain of sterol was achieved by solid-liquid phase-transfer Wittig reaction using (3-methyl-2-oxo)butyltriphenylarsonium bromide and K 2 CO 3.
12 citations
Patent•
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07 Feb 2001
TL;DR: A cell culture medium comprises stigmasterol and β-sitosterol or esters or other derivatives thereof, with ethanol, propylene glycol, Pluronic F68, Polysorbate 80, ethanolamine and acetic acid as discussed by the authors.
Abstract: A cell culture medium comprises stigmasterol and β-sitosterol or esters or other derivatives thereof, with ethanol, propylene glycol, Pluronic F68, Polysorbate 80, ethanolamine and acetic acid.
11 citations
30 Jun 2001
TL;DR: In this paper, the leaves of Ficus infectoria (Roxb.) cultivated in Egypt were isolated for the first time from the ethanolic ext. and spectral data (UV, IR, MS, 1H and 13C-NMR) as well as by comparison with authentic samples.
Abstract: α-Amyrin, stigmasterol, bergapten, stigmasterol-3-O-β-D-glucoside, kaempferol-3-O-β-D-glucoside, benzyl-glucoside, quercetin-3-O-β-D-glucoside, 6-hydroxykaempferol-7-O-β-D-glucoside, and quercetin-3-O-β-D-rutinoside were isolated for the first time from the ethanolic ext. of the leaves of Ficus infectoria (Roxb.) cultivated in Egypt. Identification of these compds. has been established by phys. and spectral data (UV, IR, MS, 1H- and 13C-NMR) as well as by comparison with authentic samples.
6 citations
Patent•
22 Mar 2001TL;DR: In this article, the authors presented a method for decreasing the blood concentration of total and LDL cholesterol in a human in which the plant sterol and a soy protein material and/or an isoflavone are co-administered to the human, where the plstol comprises at least 0.49% of the composition, by weight.
Abstract: A composition is provided comprising a plant sterol and a soy protein material and/or and isoflavone selected from genistein, daidzein, glycitein, biochanin A, formononetin, and their naturally occurring glycosides, where the plant sterol comprises at least 0.49% of the composition, by weight. The present invention is also a method for decreasing the blood concentration of total and LDL cholesterol in a human in which the plant sterol and a soy protein material and/or an isoflavone are co-administered to the human, where the plant sterol comprises at least 0.49%, by weight, of the combined weight of the plant sterol and the soy protein material and/or the isoflavone. Also provided is a method for preventing or minimizing the development of atherosclerosis in a human in which a plant sterol and a soy protein material and/or an isoflavone are co-administered to the human, where the plant sterol comprises at least 0.49%, by weight, of the combined weight of the plant sterol and the soy protein material and/or the isoflavone. A preferred method involves co-administering to a human a plant sterol and a soy protein material containing at least 0.49% by weight soy protein and containing the isoflavone glycoside, glycitin. The plant sterol is at least 0.49% by combined weight of the co-administered plant sterol and soy protein material. The plant sterol can be B-sitosterol, campesterol, stigmasterol, sitostanol, or campestanol. Also an isoflavone can be administered in combination with the plant sterol and soy protein material. This isoflavone can be genistein, daidzein, glycitein, biochanin A, formononetin, and their naturally occurring glycosides and glycoside conjugates.
Patent•
20 Jun 2001
TL;DR: The protein beverage for reducing serum cholesterol comprises 0.1-10% of phytosterol aliphatic ester which is made up by phtosterol and alphatic acid through the process of esterification as discussed by the authors.
Abstract: The protein beverage for reducing serum cholesterol comprises 0.1-10% (wt%) of phytosterol aliphatic ester which is made up by phtosterol and aliphatic acid through the process of esterification, in which the phytosterol is the mixture of beta-sitosterol, campesterol, stigmasterol and other natural sterol, and its aliphatic acid is one of C2-C22 aliphatic acids or their mixed aliphatic acid. The protein beverage containing above 0.75g of phytosterol aliphatic ester can be taken in by adult to attain the goal of reducing cholesterol to reduce incidence rate and death rate of coronary heart disease.
01 Jan 2001
TL;DR: The dichloromethane extract of Pseuderanthemum bicolor afforded lupeol, betulin and a mixture of stigmasterol and sitosterol, which inhibited the growth of the fungi, Candida albicans and Aspergillus niger, but did not exhibit any activity against Trichophyton mentagrophytes.
Abstract: The dichloromethane extract of Pseuderanthemum bicolor afforded lupeol, betulin and a mixture of stigmasterol and sitosterol. Antimicrobial tests on the extract and the isolates indicated that they were active against the gram negative bacteria, Escherichia coli and Pseudomonas aeruginosa, but inactive against the gram-positive bacteria, Staphylococcus aureus and Bacillus subtilis at 30 µg. Lupeol inhibited the growth of the fungi, Candida albicans and Aspergillus niger, but did not exhibit any activity against Trichophyton mentagrophytes. The mixture of stigmasterol and sitosterol was active against C. albicans and T. mentagrophytes, but inactive against A. niger. The extract and betulin were inactive against all the fungi tested.
Patent•
30 Nov 2001
TL;DR: In this article, the use of sterols derived from a plant or algae extract as the active principle of a cosmetic composition for combating adiposity was proposed, which are, for example, at least one of the three following sterols: campesterol, beta-sitosterol or stigmasterol.
Abstract: The present invention relates to the use of sterols derived from a plant or algae extract as the active principle of a cosmetic composition for combating adiposity. Said sterols are, for example, at least one of the three following sterols: campesterol, beta-sitosterol or stigmasterol.
Journal Article•
TL;DR: The chemical constituents of Carduus crispus were studied for the first time using chromatographic methods to isolate compounds and using chemical and spectral methods to elucidate the structures of the isolated compounds.
Abstract: Objective To study the chemical constituents of Carduus crispus. Method Using chromatographic methods to isolate compounds and using chemical and spectral methods to elucidate the structures of the isolated compounds. Result Eight compounds were elucidated as beta-amyrin palmitate, taraxastery acetate, luteolin-7-O-alpha-L-rhamanopyranosyl-(1-->2)-beta-D-glucopyranoside, luteolin-7-O-beta-D-glucopyranoside, triacontanic acid, beta-sitosterol, stigmasterol and stigmast-7-en-3 beta-ol. Conclusion All the compounds were obtained from this plant for the first time.
01 Apr 2001
TL;DR: Two new compounds, melanochromone and 2-ethoxyl-2-(4-hydroxyphenyl)ethanol, were isolated from the whole plants of Melanosoiadum pimpinelloideum II.
Abstract: Two new compounds, melanochromone and 2-ethoxyl-2-(4-hydroxyphenyl)ethanol. were isolated from the whole plants of Melanosoiadum pimpinelloideum II. Boiss. The known compounds isolated were 1-(4-hydroxyphenyl)-1.2ethanediol. tymine. cimifugin, umtatin. bergenin. daucosterol and stigmasterol. Their structures were determined on the basis of spectral data.
Patent•
30 Mar 2001TL;DR: In this paper, the authors presented a method for decreasing the blood concentration of total and LDL cholesterol in a human in which a plant sterol and a soy protein material and/or an isoflavone are co-administered to the human, where the plant sterols comprises at least 0.49% of the composition, by weight.
Abstract: A composition is provided comprising a plant sterol and a soy protein material and/or and isoflavone selected from genistein, daidzein, glycitein, biochanin A, formononetin, and their naturally occurring glycosides, where the plant sterol comprises at least 0.49% of the composition, by weight. The present invention is also a method for decreasing the blood concentration of total and LDL cholesterol in a human in which the plant sterol and a soy protein material and/or an isoflavone are co-administered to the human, where the plant sterol comprises at least 0.49%, by weight, of the combined weight of the plant sterol and the soy protein material and/or the isoflavone. Also provided is a method for preventing or minimizing the development of atherosclerosis in a human in which a plant sterol and a soy protein material and/or an isoflavone are co-administered to the human, where the plant sterol comprises at least 0.49%, by weight, of the combined weight of the plant sterol and the soy protein material and/or the isoflavone. A preferred composition contains an isoflavone material free of soy bean protein and at least 0.49% by weight of plant sterol. The isoflavone can be genistein, daidzein, glycitein, biochanin A, formononetin, and their naturally occurring glycosides and glycoside conjugates. The plant sterol can be B-sitosterol, campesterol, stigmasterol, sitostanol, or campestanol.
Journal Article•
TL;DR: In this article, seven compounds were isolated from the aerial parts of Eupatorium odoratum Linn. by means of spectral methods, their structures were respectively elucidated as four flavonoids and β sitosterol, stigmasterol, β daucosterol.
Abstract: Seven compounds were isolated from the aerial parts of Eupatorium odoratum Linn.By means of spectral methods,their structures were respectively elucidated as four flavonoids kaempferol 4 methyl ether,quercetin 7,4 dimethyl ether,acacetin,naringenin 4 methyl ether and β sitosterol,stigmasterol,β daucosterol.
Journal Article•
TL;DR: In this paper, the chemical constituents obtained from Hedysarum multijugum were separated by liquid liquid extraction and chromatography, their structures were identified by spectral analyses, and seven compounds were obtained triacontyl alcohol (1), tetracosanoic acid (2), 5,7 dihydroxy 6, 8 di C prenyl 4′ methoxy isoflavone (3), 4′ hydroxy trans cinnamic acid docosyl ester (4), stigmasterol (5); 5, 7 dihydrox 6 C pre
Abstract: To study the chemical constituents obtained from Hedysarum multijugum . Methods: The compounds were separated by liquid liquid extraction and chromatography, their structures were identified by spectral analyses. Results: Seven compounds were obtained triacontyl alcohol (1); tetracosanoic acid (2); 5,7 dihydroxy 6, 8 di C prenyl 4′ methoxy isoflavone (3); 4′ hydroxy trans cinnamic acid docosyl ester (4); stigmasterol (5); 5, 7 dihydroxy 6 C prenyl 4′ methoxy isoflavone (6, Gancaonin M); caffeic acid tetracosyl ester (7). Conclusion: Compounds 3 and 4 were new natural compounds and the rest were obtained from the plant for the first time.
Journal Article•
TL;DR: In this article, the chemical constituents of the whole plant were identified by column chromatography and spectroscopic methods, such as IR, MS and 1H NMR, and five compounds were identified as hentriantane I, taraxasteryl acetate II, stigmasterol III, beta-sitosterol IV and stig masterol V.
Abstract: Objective To separate and identify the chemical constituents of the whole plant. Method The compounds were extracted with solvents, isolated by column chromatography and identified by spectroscopic methods, such as IR, MS and 1H NMR. Result Five compounds were identified as hentriantane I, taraxasteryl acetate II, taraxasterol III, beta-sitosterol IV, and stigmasterol V. Conclusion The compounds III and V were obtained from the plant for the first time.
Journal Article•
TL;DR: Four compounds were identified as kaempferol 3 O glucopyranoside, naringin 4' glucoside, stigmasterol (III),lupeol (IV), and compound II?III?IV were obtained first time from this plant.
Abstract: Aim To study the constituents of Lysimachia clethroide Duby.Methods The constituents were isolated and purified by using column chromatography with silica gel.These compounds were identified by their physical and spectral data.Results Four compounds were identified as kaempferol 3 O glucopyranoside (I),naringin 4' glucoside (II), stigmasterol (III),lupeol (IV).Conclusion Compound II?III?IV were obtained first time from this plant.
Journal Article•
TL;DR: A preliminary study on extraction, separation and identification of chemical constituents in roots and seeds of Pueraria wallchii DC was carried out as mentioned in this paper, where five chemical compounds were obtained and preliminarily identified as the flavones: daidzein (A), daidzin(B), puerarin(C), stigmasterol (D), and β sitosterol (E).
Abstract: A preliminary study on extraction, separation and identification of chemical constituents in roots and seeds of Pueraria wallchii DC.was carried out. Five chemical compounds were obtained and preliminarily identified as the flavones: daidzein (A), daidzin(B), puerarin(C)and the steroids: stigmasterol (D) and β sitosterol (E). These provide scientific basis for the exploitation and utilization of P.wallchii DC.
TL;DR: A butanolide, marliolide, was isolated from the hexane extract of leaves of Mollinedia marliae (Monimiacae) along with the long chain fatty alcohol hexacosanol.
Abstract: A butanolide, marliolide, was isolated from the hexane extract of leaves of Mollinedia marliae (Monimiacae) along with the long chain fatty alcohol hexacosanol. Phytol and a mixture of sitosterol and stigmasterol were also isolated from leaves of both M. marliae and M. gilgiana. trans-N-Feruloyltyramine, a ferulic acid derivative, is the major constituent of the stems of M. gilgiana.
TL;DR: In this article, a new synthetic scheme was developed to synthesize 22,23-epoxyecdysteroids from stigmasterol, which can be used to produce 22.
Abstract: A new synthetic scheme has been developed to synthesize 22,23-epoxyecdysteroids from stigmasterol
Journal Article•
TL;DR: This is the first time for the chemical constituents of L. urticifolius to be reported and established as poliothrysoside, beta-sitosterol and stigmasterol, palmitic acid and tyrosine.
Abstract: Objective To investigate the chemical constituents from the whole plant of Loxocalyx urticifolius. Method The chemical constituents were isolated by chromatography and identified by MS, 1H NMR, 13C NMR and 2D-NMR methods. Result The structures were established as poliothrysoside, beta-sitosterol and stigmasterol, palmitic acid and tyrosine. Conclusion This is the first time for the chemical constituents of L. urticifolius to be reported.
01 Jan 2001
TL;DR: The dichloromethane extract of Pseuderanthemum bicolor afforded lupeol, betulin and a mixture of stigmasterol and sitosteroL and antimicrobial tests on the extract and the isolates indicated that they were active against the gram negative bacteria and Pseudomonas aeruginosa, but inactive against the Gram positive bacteria.
Abstract: 'Chemistry Department De La Salle University Manila 1004, Philippines 2Chemistry Department Adamson University Manila, Philippines 3Schoo/ of Chemical and Biomedical Sciences Central Queensland University _Queensland 4702, Australia The dichloromethane extract of Pseuderanthemum bicolor afforded lupeol, betulin and a mixture of stigmasterol and sitosteroL Antimicrobial tests on the extract and the isolates indicated that they were active against the gram negative bacteria, Escherichia coli and Pseudomonas aeruginosa, but inactive against the gram-positive bacteria, Staphylococcus aureus and Bacillus subtilis at 30 J.tg. Lupeol inhibited the growth of the fungi, Candida albicans and Aspergillus niger, but did not exhibit any activity against Trichophyton mentagrophytes. The mixture of stigmasterol and sitosterol was active against C. albicans and T. mentagrophytes, but inactive against A. niger. The extract and betulin were inactive against all the fungi tested. Keywords: Pseuderanthemum bicolor; Acanthaceae; lupeol; betulin; stigmasterol; sitosterol; antimicrobial
Patent•
04 Apr 2001TL;DR: In this paper, the authors presented a method for decreasing the blood concentration of total and LDL cholesterol in a human in which a plant sterol and a soy protein material and/or an isoflavone are co-administered to the human, where the plant sterols comprises at least 0.49% of the composition, by weight.
Abstract: A composition is provided comprising a plant sterol and a soy protein material and/or and isoflavone selected from genistein, daidzein, glycitein, biochanin A, formononetin, and their naturally occurring glycosides, where the plant sterol comprises at least 0.49% of the composition, by weight. The present invention is also a method for decreasing the blood concentration of total and LDL cholesterol in a human in which the plant sterol and a soy protein material and/or an isoflavone are co-administered to the human, where the plant sterol comprises at least 0.49%, by weight, of the combined weight of the plant sterol and the soy protein material and/or the isoflavone. Also provided is a method for preventing or minimizing the development of atherosclerosis in a human in which a plant sterol and a soy protein material and/or an isoflavone are co-administered to the human, where the plant sterol comprises at least 0.49%, by weight, of the combined weight of the plant sterol and the soy protein material and/or the isoflavone. A preferred composition contains soy hypocotyl material and at least 0.49% by weight plant sterol. The plant sterol can be B-sitosterol, campesterol, stigmasterol, sitostanol, or campestanol.
31 Aug 2001
TL;DR: Using electron and visible microscopy, it is shown that the mated pairs in stigmasterol remained adherent but prezygotic even after 12 hours incubation, and it was clear that membrane fusion was perturbed by sterol alterations.
Abstract: : Virtually every antifungal agent in use intercedes some aspect of sterol synthesis or function. Ergosterol is the principal sterol in fungi, while cholesterol is the most abundant sterol in vertebrates. Research in our laboratory has shown that the structural differences in ergosterol, in comparison to cholesterol, have distinctive biochemical and physiological effects in fungi. Under the completed contract we have shown that as the sterol composition of sterol auxotrophic strains of Saccharomyces cerevisiae is altered there is disturbance of the mating efficiency of the strains. The normal sterol, ergosterol, mediates a 30-fold higher productive mating efficiency in the auxotrophs than when the cells are supplied with stigmasterol. Using electron and visible microscopy, we have shown that the mated pairs in stigmasterol remained adherent but prezygotic even after 12 hours incubation. Ergosterol rescued the cells and permitted zygote formation. Based on those experiments it was clear that membrane fusion was perturbed by sterol alterations. Continuing work with the renewal grant is focusing on membrane fusion in various normal cell biological processes.