Aaron Klammer

TL;DR: This work presents a hierarchical genome-assembly process (HGAP) for high-quality de novo microbial genome assemblies using only a single, long-insert shotgun DNA library in conjunction with Single Molecule, Real-Time (SMRT) DNA sequencing.

TL;DR: The findings suggest that horizontal genetic exchange allowed for the emergence of the highly virulent Shiga-toxin-producing enteroaggregative E. coli O104:H4 strain that caused the German outbreak, and highlight the way in which the plasticity of bacterial genomes facilitates the emerged of new pathogens.

TL;DR: This work combines sequencing data from second- and third-generation DNA sequencing technologies to assemble the two-chromosome genome of a recent Haitian cholera outbreak strain into two nearly finished contigs at >99.9% accuracy and provides the next generation of rapid microbial identification and full-genome assembly.

TL;DR: In particular preferred embodiments, nucleic acid sequences generated by such methods are subjected to de novo assembly and/or consensus sequence determination as mentioned in this paper, which is a common problem in sequence-by-incorporation processes.

TL;DR: In this article, a hierarchical genome assembly process for producing high-quality de novo genome assemblies is presented, which utilizes a single, long-insert, shotgun DNA library in conjunction with Single Molecule, Real-Time (SMRT) DNA sequencing, and obviates the need for additional sample preparation and sequencing data sets required for previously described hybrid assembly strategies.