Lawrence Lee

TL;DR: The findings suggest that horizontal genetic exchange allowed for the emergence of the highly virulent Shiga-toxin-producing enteroaggregative E. coli O104:H4 strain that caused the German outbreak, and highlight the way in which the plasticity of bacterial genomes facilitates the emerged of new pathogens.

TL;DR: How to master the different types of computational environments that exist — such as cloud and heterogeneous computing — to successfully tackle the authors' big data problems is discussed.

TL;DR: A computational method, LSC, to perform error correction of TGS long reads (LR) by SGS short reads (SR) by applying a homopolymer compression (HC) transformation strategy to increase the sensitivity of SR-LR alignment without scarifying alignment accuracy is presented.

TL;DR: A new method based on the combined analysis of short reads and long reads generated by second- and third-generation sequencing and a custom-designed method for isoform identification and quantification is developed to generate a high-confidence isoform dataset for human embryonic stem cells (hESCs).

TL;DR: All four NGS platforms were able to detect minority non-R5 variants at comparable levels suggesting that any NGS-based method can be used to predict HIV-1 coreceptor usage.