James P Drake

TL;DR: This work presents a hierarchical genome-assembly process (HGAP) for high-quality de novo microbial genome assemblies using only a single, long-insert shotgun DNA library in conjunction with Single Molecule, Real-Time (SMRT) DNA sequencing.

TL;DR: The MinHash Alignment Process (MHAP) is introduced for overlapping noisy, long reads using probabilistic, locality-sensitive hashing and can produce de novo near-complete eukaryotic assemblies that are 99.99% accurate when compared with available reference genomes.

TL;DR: The MinHash Alignment Process (MHAP) is introduced for efficient overlapping of noisy, long reads using probabilistic, locality-sensitive hashing and demonstrated that single-molecule sequencing alone can produce near-complete eukaryotic genomes at modest cost.

TL;DR: The quality of the Asian seabass genome assembly far exceeds that of any other fish species, and will serve as a new standard for fish genomics.

TL;DR: In the version of this article initially published, equation 9 appeared incorrectly as: The equation has been corrected in the HTML and PDF versions of the article.