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Alfredo Gonzalez
Researcher at Broad Institute
Publications - Â 8
Citations - Â 373
Alfredo Gonzalez is an academic researcher from Broad Institute. The author has contributed to research in topics: Synthetic lethality & Cancer. The author has an hindex of 3, co-authored 6 publications receiving 167 citations.
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Journal ArticleDOI
WRN helicase is a synthetic lethal target in microsatellite unstable cancers.
Edmond M. Chan,Edmond M. Chan,Tsukasa Shibue,James M. McFarland,Benjamin Gaeta,Mahmoud Ghandi,Nancy Dumont,Alfredo Gonzalez,Justine S. McPartlan,Tianxia Li,Yanxi Zhang,Jie Bin Liu,Jean-Bernard Lazaro,Peili Gu,Cortt G. Piett,Annie Apffel,Syed O. Ali,Syed O. Ali,Rebecca Deasy,Paula Keskula,Raymond W.S. Ng,Raymond W.S. Ng,Emma A. Roberts,Elizaveta Reznichenko,Lisa Leung,Maria Alimova,Monica Schenone,Mirazul Islam,Mirazul Islam,Yosef E. Maruvka,Yosef E. Maruvka,Yang Liu,Yang Liu,Jatin Roper,Srivatsan Raghavan,Srivatsan Raghavan,Marios Giannakis,Marios Giannakis,Yuen-Yi Tseng,Zachary D. Nagel,Zachary D. Nagel,Alan D. D'Andrea,David E. Root,Jesse S. Boehm,Gad Getz,Gad Getz,Sandy Chang,Todd R. Golub,Todd R. Golub,Todd R. Golub,Aviad Tsherniak,Francisca Vazquez,Francisca Vazquez,Adam J. Bass,Adam J. Bass +54 more
TL;DR: Data from large-scale silencing screens using CRISPR–Cas9-mediated knockout and RNA interference found that the RecQ DNA helicase WRN was selectively essential in MSI models in vitro and in vivo, yet dispensable in models of cancers that are microsatellite stable.
Posted ContentDOI
WRN Helicase is a Synthetic Lethal Target in Microsatellite Unstable Cancers
Edmond M. Chan,Edmond M. Chan,Tsukasa Shibue,James M. McFarland,Benjamin Gaeta,Justine S. McPartlan,Mahmoud Ghandi,Jie Bin Liu,Jean-Bernard Lazaro,Nancy Dumont,Alfredo Gonzalez,Annie Apffel,Syed O. Ali,Syed O. Ali,Lisa Leung,Emma A. Roberts,Elizaveta Reznichenko,Mirazul Islam,Mirazul Islam,Maria Alimova,Monica Schenone,Yosef E. Maruvka,Yang Liu,Yang Liu,Alan D. D'Andrea,David E. Root,Jesse S. Boehm,Gad Getz,Todd R. Golub,Aviad Tsherniak,Francisca Vazquez,Adam J. Bass,Adam J. Bass +32 more
TL;DR: Analysis of data from large-scale CRISPR/Cas9 knockout and RNA interference silencing screens found that the RecQ DNA helicase WRN was selectively essential in MSI cell lines, yet dispensable in microsatellite stable (MSS) cell lines.
Journal ArticleDOI
Synthetic Lethal Interaction of SHOC2 Depletion with MEK Inhibition in RAS-Driven Cancers
Rita Sulahian,Jason J. Kwon,Jason J. Kwon,Katherine H. Walsh,Emma Pailler,Emma Pailler,Timothy L. Bosse,Maneesha Thaker,Diego Almanza,Joshua M. Dempster,Joshua Pan,Joshua Pan,Federica Piccioni,Nancy Dumont,Alfredo Gonzalez,Jonathan P. Rennhack,Jonathan P. Rennhack,Behnam Nabet,John A. Bachman,Amy Goodale,Yenarae Lee,Mukta Bagul,Rosy Liao,Adrija J. Navarro,Tina L. Yuan,Raymond W.S. Ng,Srivatsan Raghavan,Srivatsan Raghavan,Nathanael S. Gray,Aviad Tsherniak,Francisca Vazquez,David E. Root,Ari J. Firestone,Jeff Settleman,William C. Hahn,Andrew J. Aguirre +35 more
TL;DR: It is demonstrated that knockout, suppression, or degradation of SHOC2, a positive regulator of MAPK signaling, specifically cooperated with MEK inhibition to impair proliferation in RAS-driven cancer cells.
Journal ArticleDOI
Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q.
Jasper E. Neggers,Jasper E. Neggers,Brenton R. Paolella,Brenton R. Paolella,Adhana Asfaw,Michael V. Rothberg,Thomas A. Skipper,Annan Yang,Radha L. Kalekar,Radha L. Kalekar,John M. Krill-Burger,Neekesh V. Dharia,Neekesh V. Dharia,Neekesh V. Dharia,Guillaume Kugener,Jeremie Kalfon,Chen Yuan,Nancy Dumont,Alfredo Gonzalez,Mai Abdusamad,Yvonne Y. Li,Yvonne Y. Li,Liam F. Spurr,Liam F. Spurr,Westley W. Wu,Westley W. Wu,Adam D. Durbin,Adam D. Durbin,Adam D. Durbin,Brian M. Wolpin,Federica Piccioni,David E. Root,Jesse S. Boehm,Andrew D. Cherniack,Andrew D. Cherniack,Aviad Tsherniak,Andrew L. Hong,Andrew L. Hong,Andrew L. Hong,William C. Hahn,William C. Hahn,Kimberly Stegmaier,Kimberly Stegmaier,Kimberly Stegmaier,Todd R. Golub,Todd R. Golub,Francisca Vazquez,Francisca Vazquez,Andrew J. Aguirre,Andrew J. Aguirre +49 more
TL;DR: A compendium of synthetic lethal vulnerabilities is described and V PS4A and VPS4B are nominated as high-priority therapeutic targets for cancers with 18q or 16q loss.
Posted ContentDOI
Phosphate dysregulation via the XPR1:KIDINS220 protein complex is a therapeutic vulnerability in ovarian cancer
Daniel P. Bondeson,Brenton R. Paolella,Adhana Asfaw,Michael V. Rothberg,Thomas A. Skipper,Carly Langan,Alfredo Gonzalez,Lauren E. Surface,Kentaro Ito,Mariya Kazachkova,William Colgan,Allison Warren,Josh Dempster,Mike Burger,Maria Ericsson,Andrew Tang,Iris Fung,Emily Chambers,Mai Abdusamad,Nancy Dumont,John G. Doench,Federica Piccioni,David E. Root,Jesse S. Boehm,William C. Hahn,William C. Hahn,Michael Mannstadt,James M. McFarland,Francisca Vazquez,Todd R. Golub,Todd R. Golub +30 more
TL;DR: All data point to the XPR1:KIDINS220 complex - and phosphate dysregulation more broadly - as a therapeutic vulnerability in ovarian cancer.