L
Lukasz Sedek
Researcher at Medical University of Silesia
Publications - 26
Citations - 3501
Lukasz Sedek is an academic researcher from Medical University of Silesia. The author has contributed to research in topics: Minimal residual disease & Immunophenotyping. The author has an hindex of 15, co-authored 24 publications receiving 2708 citations. Previous affiliations of Lukasz Sedek include University of Silesia in Katowice.
Papers
More filters
Journal ArticleDOI
EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes.
J. J. M. Van Dongen,Ludovic Lhermitte,S Böttcher,Julia Almeida,V H J van der Velden,Juan Flores-Montero,Andy C. Rawstron,Vahid Asnafi,Quentin Lecrevisse,Paulo Sérgio Lucio,Ester Mejstrikova,Tomasz Szczepański,Tomas Kalina,R de Tute,Monika Brüggemann,Lukasz Sedek,M Cullen,Anthonie Willem Langerak,Alexandre de Mendonça,Elizabeth Macintyre,Marta Martin-Ayuso,Ondrej Hrusak,M B Vidriales,Alberto Orfao +23 more
TL;DR: The EuroFlow studies resulted in validated and flexible 8-color antibody panels for multidimensional identification and characterization of normal and aberrant cells, optimally suited for immunophenotypic screening and classification of hematological malignancies.
Journal ArticleDOI
EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols
Tomas Kalina,Juan Flores-Montero,V H J van der Velden,Marta Martin-Ayuso,S Böttcher,Matthias Ritgen,Julia Almeida,Ludovic Lhermitte,Vahid Asnafi,Alexandre de Mendonça,R de Tute,M Cullen,Lukasz Sedek,M B Vidriales,J.J. Pérez,J. G. te Marvelde,Ester Mejstrikova,Ondrej Hrusak,T Szczepa nacute,ski,J. J. M. Van Dongen,Alberto Orfao +21 more
TL;DR: The 6 years of extensive collaborative experiments and the analysis of hundreds of cell samples of patients and healthy controls have provided for the first time laboratory protocols and software tools for fully standardized 8-color flow cytometric immunophenotyping of normal and malignant leukocytes in bone marrow and blood; this has yielded highly comparable data sets, which can be integrated in a single database.
Journal ArticleDOI
The MLL recombinome of acute leukemias in 2017
Christian Meyer,Thomas Burmeister,Daniela Gröger,Grigory Tsaur,L Fechina,Aline Renneville,Rosemary Sutton,Nicola C. Venn,Mariana Emerenciano,Maria S. Pombo-de-Oliveira,C Barbieri Blunck,B Almeida Lopes,Jan Zuna,Jan Trka,Paola Ballerini,Hélène Lapillonne,M. De Braekeleer,Giovanni Cazzaniga,L Corral Abascal,V H J van der Velden,Eric Delabesse,T S Park,S H Oh,Maria Luiza Macedo Silva,T Lund-Aho,Vesa Juvonen,Andrew S. Moore,Olaf Heidenreich,Josef Vormoor,Elena Zerkalenkova,Yulia Olshanskaya,Clara Bueno,Clara Bueno,Pablo Menendez,Pablo Menendez,Andrea Teigler-Schlegel,U zur Stadt,Jana Lentes,Gudrun Göhring,Anatoly Kustanovich,Olga Aleinikova,Beat W. Schäfer,S Kubetzko,Hans O. Madsen,Bernd Gruhn,X Duarte,Paula Gameiro,Eric Lippert,A Bidet,J M Cayuela,Emmanuelle Clappier,Cristina N. Alonso,Christian M. Zwaan,M M van den Heuvel-Eibrink,Shai Izraeli,Shai Izraeli,L. Trakhtenbrot,L. Trakhtenbrot,P Archer,Jeremy Hancock,Anja Möricke,Julia Alten,Martin Schrappe,Martin Stanulla,Sabine Strehl,Andishe Attarbaschi,Michael Dworzak,Oskar A. Haas,Renate Panzer-Grümayer,Lukasz Sedek,Tomasz Szczepański,Aurélie Caye,L Suarez,Hélène Cavé,Rolf Marschalek +74 more
TL;DR: This study provides a comprehensive analysis of the MLL recombinome in acute leukemia and demonstrates that the establishment of patient-specific chromosomal fusion sites allows the design of specific PCR primers for minimal residual disease analyses for all patients.
Journal ArticleDOI
Next Generation Flow for highly sensitive and standardized detection of minimal residual disease in multiple myeloma
Juan Flores-Montero,Luzalba Sanoja-Flores,Bruno Paiva,N. Puig,O García-Sánchez,S Böttcher,V H J van der Velden,J-J Pérez-Morán,M B Vidriales,Ramón García-Sanz,Carmen Jiménez,Marcos González,Joaquin Martinez-Lopez,Alba Corral-Mateos,G-E Grigore,Rafael Fluxa,Roberia Pontes,Joana Caetano,Lukasz Sedek,M-C del Cañizo,J. Bladé,J. J. Lahuerta,Carlos Aguilar,Abelardo Bárez,Aránzazu García-Mateo,Jorge Labrador,Pilar Leoz,C Aguilera-Sanz,Jesús F. San-Miguel,M.V. Mateos,Brian G.M. Durie,J. J. M. Van Dongen,J. J. M. Van Dongen,Alberto Orfao +33 more
TL;DR: EuroFlow-based NGF is established as a highly sensitive, fully standardized approach for MRD detection in MM which overcomes the major limitations of conventional flow-MRD methods and is ready for implementation in routine diagnostics.
Journal ArticleDOI
The MLL recombinome of acute leukemias in 2013
Claus Meyer,Julia Hofmann,Thomas Burmeister,Daniela Gröger,T S Park,Mariana Emerenciano,M. Pombo De Oliveira,Aline Renneville,Patrick Villarese,Elizabeth Macintyre,Hélène Cavé,Emmanuelle Clappier,K. Mass-Malo,Jan Zuna,Jan Trka,E De Braekeleer,M. De Braekeleer,S H Oh,Grigory Tsaur,L Fechina,V H J van der Velden,J J M van Dongen,Eric Delabesse,Renata Binato,Mara Silva,AM Kustanovich,Olga V. Aleinikova,Marian H. Harris,T Lund-Aho,Vesa Juvonen,Olaf Heidenreich,Josef Vormoor,William W.L. Choi,Marie Jarošová,A. Kolenova,Clara Bueno,Pablo Menendez,S. Wehner,Cornelia Eckert,Pascaline Talmant,Sylvie Tondeur,Eric Lippert,E. Launay,Catherine Henry,Paola Ballerini,H. Lapillone,Mary Callanan,Jean Michel Cayuela,Charles Herbaux,Giovanni Cazzaniga,P. M. Kakadiya,Stefan K. Bohlander,Martina Ahlmann,Jong Rak Choi,Paula Gameiro,Dongsoon Lee,Juergen Krauter,Pascale Cornillet-Lefebvre,G te Kronnie,Beat W. Schäfer,S. Kubetzko,Cristina N. Alonso,U. Zur Stadt,Rosemary Sutton,N. C. Venn,Shai Izraeli,Luba Trakhtenbrot,H. O. Madsen,P. Archer,Jeremy Hancock,Nuno Cerveira,Manuel R. Teixeira,L Lo Nigro,Anja Möricke,Martin Stanulla,Martin Schrappe,Lukasz Sedek,Tomasz Szczepański,Christian M. Zwaan,Eva A. Coenen,M.M. van den Heuvel-Eibrink,Sabine Strehl,Michael Dworzak,Renate Panzer-Grümayer,Theodor Dingermann,Thomas Klingebiel,Rolf Marschalek +86 more
TL;DR: Long-distance inverse-polymerase chain reaction was used to characterize the chromosomal rearrangement of individual acute leukemia patients and revealed a total of 121 different MLL rearrangements, of which 79 TPGs are now characterized at the molecular level.