Christian Klose

TL;DR: A shotgun-based lipidomics workflow was developed that enabled the absolute quantification of mammalian membrane lipidomes with minimal sample processing from low sample amounts and investigated the remodeling of the total cell membrane lipidome during the transition from a nonpolarized morphology to an epithelial morphology and vice versa.

TL;DR: The central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, and it was also initiated to stimulate a discussion regarding areas in need of improvement.

TL;DR: Investigation of the influence of a variety of commonly used growth conditions on the yeast lipidome allowed for a quantitative description of the intrinsic flexibility of a eukaryotic lipidome, thereby providing new insights into the adjustments of lipid biosynthetic pathways.

TL;DR: This shotgun lipidomics platform can be implemented in different laboratories without compromising reproducibility, allowing multi-site studies and inter-laboratory comparisons, and achieves absolute quantification, by inclusion of internal standards for every lipid class measured.

TL;DR: The formation of E-Syt-dependent ER–PM tethers in response to stimuli that cleave PtdIns(4,5)P2 and elevate Ca2+ may help reverse accumulation of diacylglycerol in the PM by transferring it to the ER for metabolic recycling.