Christina M. Woo

TL;DR: Alkyl diazirines are an especially effective chemistry for surveying the membrane proteome and will facilitate design and interpretation of biomolecular labeling experiments with diazIRines, according to a systematic evaluation of the labeling preferences.

TL;DR: These first proximity-directed OGT constructs provide a flexible strategy for targeting additional proteins and a template for further engineering of OGT and the O-GlcNAc proteome in the future.

TL;DR: Using SIM-PAL, the NSAID interactome consisting of over 1000 significantly enriched proteins was identified and nearly 200 conjugated peptides representing direct binding sites of the photo-NSAIDs with proteins from Jurkat and K562 cells were characterized.

TL;DR: In this article, a community study, comprising both developers and users of glycoproteomics software, evaluates solutions for system-wide glycopeptide analysis and concludes that diverse software packages for comprehensive glyopeptides data analysis exist, points to several high-performance search strategies and specifies key variables that will guide future software developments and assist informatics decision-making in glycoproteinomics.

TL;DR: These findings support a model where sites of glycosylation for many OGT substrates are determined by TPR domain contacts to substrate side chains five to fifteen residues C-terminal to the glycosite, and inform efforts to engineer substrates to explore biological functions.