Elisa D’Este

TL;DR: Far-red, fluorogenic probes are introduced that reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.

TL;DR: It is shown that the periodic subcortical actin structure is in fact present in both axons and dendrites, and also found in the peripheral nervous system, specifically at the nodes of Ranvier.

TL;DR: This work shows that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently superimposed orthogonal standing light waves, providing isotropic resolution in the focal plane and making pattern rotation redundant.

TL;DR: A far-red DNA stain, SiR–Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy, which makes this probe a powerful tool for live-cell imaging.

TL;DR: In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding the capacity for imaging living cells.