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Elisa D’Este
Researcher at Max Planck Society
Publications - 57
Citations - 3380
Elisa D’Este is an academic researcher from Max Planck Society. The author has contributed to research in topics: STED microscopy & Chemistry. The author has an hindex of 20, co-authored 47 publications receiving 2472 citations. Previous affiliations of Elisa D’Este include AREA Science Park.
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Journal ArticleDOI
Fluorogenic probes for live-cell imaging of the cytoskeleton.
Gražvydas Lukinavičius,Luc Reymond,Elisa D’Este,Anastasiya Masharina,Fabian Göttfert,Haisen Ta,Angelika Güther,Mathias Fournier,Stefano Rizzo,Herbert Waldmann,Claudia Blaukopf,Christoph Sommer,Daniel W. Gerlich,Hans-Dieter Arndt,Stefan W. Hell,Kai Johnsson +15 more
TL;DR: Far-red, fluorogenic probes are introduced that reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.
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STED Nanoscopy Reveals the Ubiquity of Subcortical Cytoskeleton Periodicity in Living Neurons
TL;DR: It is shown that the periodic subcortical actin structure is in fact present in both axons and dendrites, and also found in the peripheral nervous system, specifically at the nodes of Ranvier.
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Nanoscopy with more than 100,000 'doughnuts'
Andriy Chmyrov,Jan Keller,Tim Grotjohann,Michael Ratz,Elisa D’Este,Stefan Jakobs,Christian Eggeling,Stefan W. Hell +7 more
TL;DR: This work shows that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently superimposed orthogonal standing light waves, providing isotropic resolution in the focal plane and making pattern rotation redundant.
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SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy
Gražvydas Lukinavičius,Claudia Blaukopf,Elias Pershagen,Alberto Schena,Luc Reymond,Emmanuel Derivery,Marcos González-Gaitán,Elisa D’Este,Stefan W. Hell,Daniel W. Gerlich,Kai Johnsson +10 more
TL;DR: A far-red DNA stain, SiR–Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy, which makes this probe a powerful tool for live-cell imaging.
Journal ArticleDOI
Fluorogenic Probes for Multicolor Imaging in Living Cells.
Gražvydas Lukinavičius,Luc Reymond,Keitaro Umezawa,Olivier Sallin,Elisa D’Este,Fabian Göttfert,Haisen Ta,Stefan W. Hell,Yasuteru Urano,Kai Johnsson +9 more
TL;DR: In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding the capacity for imaging living cells.