Emelie Berglund

TL;DR: Intra-tumor heterogeneity is one of the biggest challenges in cancer treatment today and here, the authors investigate transcriptional heterogeneity in prostate cancer, examining expression profiles of different tissue components and highlighting expression gradients in the tumor microenvironment.

TL;DR: In this paper , the authors used spatially resolved transcriptomics to infer spatial copy number variations in 120,000 regions across multiple organs, in benign and malignant tissues, and demonstrate that genome-wide copy number variation reveals distinct clonal patterns within tumours and in nearby benign tissue using an organ-wide approach focused on the prostate.

TL;DR: The method enables use of widely available short-read-sequencing platforms to study long single molecules within a complex sample, without losing phase information.

TL;DR: This approach increases the number of samples processed per run, reduces sample preparation time by 35%, and minimizes batch effects between samples, and is also shown to be highly accurate and almost completely free from technical variability between prepared samples.

TL;DR: An unsupervised approach to study spatial genome integrity in situ to describe previously unidentified clonal relationships is provided and the results suggest a model for how genomic instability arises in histologically benign tissue that may represent early events in cancer evolution.