H
Hila Epstein-Barash
Researcher at Alnylam Pharmaceuticals
Publications - 11
Citations - 2578
Hila Epstein-Barash is an academic researcher from Alnylam Pharmaceuticals. The author has contributed to research in topics: Self-healing hydrogels & Small interfering RNA. The author has an hindex of 10, co-authored 11 publications receiving 2191 citations. Previous affiliations of Hila Epstein-Barash include Massachusetts Institute of Technology & Harvard University.
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Journal ArticleDOI
Image-based analysis of lipid nanoparticle–mediated siRNA delivery, intracellular trafficking and endosomal escape
Jerome Gilleron,William Querbes,Anja Zeigerer,Anna Borodovsky,Giovanni Marsico,Undine Schubert,Kevin Manygoats,Sarah Seifert,Cordula Andree,Martin Stöter,Hila Epstein-Barash,Ligang Zhang,Victor Koteliansky,Kevin Fitzgerald,Eugenio Fava,Marc Bickle,Yannis Kalaidzidis,Akin Akinc,Martin Maier,Marino Zerial +19 more
TL;DR: It is estimated that escape of siRNAs from endosomes into the cytosol occurs at low efficiency (1–2%) and only during a limited window of time when the LNPs reside in a specific compartment sharing early and late endosomal characteristics.
Journal ArticleDOI
Therapeutic siRNA silencing in inflammatory monocytes in mice
Florian Leuschner,Partha Dutta,Rostic Gorbatov,Tatiana Novobrantseva,Jessica S. Donahoe,Gabriel Courties,Kang Mi Lee,James I. Kim,James F. Markmann,Brett Marinelli,Peter Panizzi,Won Woo Lee,Yoshiko Iwamoto,Stuart Milstein,Hila Epstein-Barash,William Cantley,Jamie Wong,Virna Cortez-Retamozo,Andita Newton,Kevin T. Love,Peter Libby,Mikael J. Pittet,Filip K. Swirski,Victor Koteliansky,Robert Langer,Ralph Weissleder,Daniel G. Anderson,Matthias Nahrendorf +27 more
TL;DR: An optimized lipid nanoparticle and a CCR2-silencing short interfering RNA that, when administered systemically in mice, show rapid blood clearance, accumulate in spleen and bone marrow, and localize to monocytes prevent their accumulation in sites of inflammation.
Journal ArticleDOI
Rab5 is necessary for the biogenesis of the endolysosomal system in vivo
Anja Zeigerer,Jerome Gilleron,Roman L. Bogorad,Giovanni Marsico,Hidenori Nonaka,Sarah Seifert,Hila Epstein-Barash,Satya Kuchimanchi,Chang Geng Peng,Vera M. Ruda,Perla Del Conte-Zerial,Jan G. Hengstler,Yannis Kalaidzidis,Victor Koteliansky,Victor Koteliansky,Marino Zerial +15 more
TL;DR: A mathematical model of endosome dependency on Rab5 was developed and validated by titrating down all three Rab5 isoforms in adult mouse liver using state-of-the-art RNA interference technology and found the endocytic system was resilient to depletion of Rab5 and collapsed only when Rab5 decreased to a critical level.
Journal ArticleDOI
A microcomposite hydrogel for repeated on-demand ultrasound-triggered drug delivery
Hila Epstein-Barash,Gizem Orbey,Baris E. Polat,Randy H. Ewoldt,Jameel A. Feshitan,Robert Langer,Mark A. Borden,Daniel S. Kohane +7 more
TL;DR: An injectable composite system based on an in situ-cross-linking hydrogel maintains model drug (dye)-containing liposomes in close proximity to gas-filled microbubbles that serve to enhance release events induced by ultrasound application.
PatentDOI
Prolonged duration local anesthesia with minimal toxicity
TL;DR: In this article, the authors used liposomes for increased loading of the site 1 sodium channel blocker, producing prolonged duration of block without systemic toxicity, which is enhanced by the addition of a corticosteroid.