Yannis Kalaidzidis

TL;DR: This work found that the level of Rab 5 dynamically fluctuates on individual early endosomes, linked by fusion and fission events into a network in time, and suggested Rab conversion as the mechanism of cargo progression between early and late endosome.

TL;DR: It is estimated that escape of siRNAs from endosomes into the cytosol occurs at low efficiency (1–2%) and only during a limited window of time when the LNPs reside in a specific compartment sharing early and late endosomal characteristics.

TL;DR: Although no single method performed best across all scenarios, the results revealed clear differences between the various approaches, leading to notable practical conclusions for users and developers.

TL;DR: It is shown that MCAK moves along the microtubule lattice in a one-dimensional (1D) random walk, corresponding to a “reduction in dimensionality” that has been proposed as the search strategy by which DNA enzymes find specific binding sites.

TL;DR: A new strategy to phenotypically profile the human genome with respect to transferrin (TF) and epidermal growth factor (EGF) endocytosis is developed by combining RNA interference, automated high-resolution confocal microscopy, quantitative multiparametric image analysis and high-performance computing.