H
Hans J. Tanke
Researcher at Leiden University
Publications - 56
Citations - 4377
Hans J. Tanke is an academic researcher from Leiden University. The author has contributed to research in topics: Fluorescence loss in photobleaching & Gene mapping. The author has an hindex of 29, co-authored 56 publications receiving 4238 citations. Previous affiliations of Hans J. Tanke include Erasmus University Rotterdam.
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Journal ArticleDOI
Heterogeneity in Telomere Length of Human Chromosomes
Peter M. Lansdorp,Nico P. Verwoerd,Frans M. van de Rijke,Visia Dragowska,Marie-Terese Little,Roeland W. Dirks,Anton K. Raap,Hans J. Tanke +7 more
TL;DR: Telomere fluorescence intensity values from metaphase chromosomes of cultured human hematopoietic cells decreased with the replication history of the cells, varied up to six-fold within a metaphase, and were similar between sister chromatid telomeres.
Journal ArticleDOI
Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy
TL;DR: Both the theoretical simulation and experimental data show that photobleaching of fluorescein in microscopy is, in general, not a single-exponential process.
Journal ArticleDOI
Telomeres in the mouse have large inter-chromosomal variations in the number of T2AG3 repeats
J. M. J. M. Zijlmans,U. M. Martens,Steven S.S. Poon,Anton K. Raap,Hans J. Tanke,Rabab K. Ward,Peter M. Lansdorp +6 more
TL;DR: It is estimated that the shortest telomeres are around 10 kb in length, indicating that each mouse cell has a few telomerres with (T2AG3)n lengths within the range of human telomere lengths, which may be critical in limiting the replicative potential of murine cells.
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Influence of the triplet excited state on the photobleaching kinetics of fluorescein in microscopy
TL;DR: Experiments demonstrated that a thiol-containing reducing agent, mercaptoethylamine (MEA or cysteamine), was the most effective, among other commonly known radical quenchers or singlet oxygen scavengers, in suppressing photobleaching of fluorescein while not reducing the fluorescence quantum yield.
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Distinct uptake mechanisms but similar intracellular processing of two different toll-like receptor ligand-peptide conjugates in dendritic cells.
Selina Khan,Martijn S. Bijker,Jimmy J. Weterings,Hans J. Tanke,Gosse J. Adema,Thorbald van Hall,Jan W. Drijfhout,Cornelis J. M. Melief,Hermen S. Overkleeft,Gijsbert A. van der Marel,Dmitri V. Filippov,Sjoerd H. van der Burg,Ferry Ossendorp +12 more
TL;DR: The data show that targeting to two distinct TLRs requires distinct uptake mechanism but follows similar trafficking and intracellular processing pathways leading to optimal antigen presentation and T-cell priming.