Influence of the triplet excited state on the photobleaching kinetics of fluorescein in microscopy
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TLDR
Experiments demonstrated that a thiol-containing reducing agent, mercaptoethylamine (MEA or cysteamine), was the most effective, among other commonly known radical quenchers or singlet oxygen scavengers, in suppressing photobleaching of fluorescein while not reducing the fluorescence quantum yield.About:
This article is published in Biophysical Journal.The article was published on 1996-06-01 and is currently open access. It has received 256 citations till now. The article focuses on the topics: Photobleaching & Singlet oxygen.read more
Citations
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Journal ArticleDOI
Size-dependent DNA Mobility in Cytoplasm and Nucleus
Gergely L. Lukacs,Peter M. Haggie,Olivier Seksek,Delphine Lechardeur,Neal D. Freedman,Alan S. Verkman +5 more
TL;DR: The results suggest that the highly restricted diffusion of DNA fragments in nucleoplasm results from extensive binding to immobile obstacles and that the decreased lateral mobility of DNAs >250 bp in cytoplasm is because of molecular crowding.
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Photobleaching of Fluorescent Dyes under Conditions Used for Single-Molecule Detection: Evidence of Two-Step Photolysis.
TL;DR: From this model, optimal conditions for SMD with respect to the number of emitted fluorescence photons and to the signal-to-background ratio are discussed, taking into account both saturation and photobleaching.
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Advances in Single-Molecule Fluorescence Methods for Molecular Biology
TL;DR: In this exciting era of transitioning from in vitro to in vivo and in situ conditions, it is anticipated that SM fluorescence methodology will become a common tool of molecular biology.
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Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion.
TL;DR: Measurements of GFP translation and rotation in aqueous dextran solutions support the view that the primary barrier to GFP diffusion is collisional interactions between GFP and macromolecular solutes.
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Solute and macromolecule diffusion in cellular aqueous compartments
TL;DR: This review evaluates experimental evidence defining the rates and barriers for molecular diffusion in cells and possible implications of regulated molecular diffusion as a rate-limiting step in cell metabolism, and with respect to the delivery of therapeutic agents are discussed.
References
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Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.
TL;DR: The theoretical basis and some practical guidelines for simple, rigorous analysis of FPR experiments are presented and some model experiments on aqueous solutions of rhodamine 6G are described.
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Fading of immunofluorescence during microscopy: a study of the phenomenon and its remedy
TL;DR: Study of the kinetics of this photo-bleaching performed by microfluorimetry and on FITC conjugate in solution and the effects of a range of additives incorporated in the mountant suggest that the fading is due to a destructive reaction with protein of the dye in its excited singlet state and that retarding additives suppress this.
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Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy
TL;DR: Both the theoretical simulation and experimental data show that photobleaching of fluorescein in microscopy is, in general, not a single-exponential process.
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Phosphorescence and the Triplet State
Gilbert N. Lewis,M. Kasha +1 more
TL;DR: In this article, the rotation of 17-isopregnan-3-a-oI-20-one with methanol was reported to be [a]*'D -28.2 * 8' (24.6 * 5'' (C Y ~ ~ D $1.34 * 0.1''; 1 = 2 dm.).
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Quantum yield of singlet oxygen production by xanthene derivatives
TL;DR: In this article, the triplet oxygen quantum yield of 11 purified fluorescein derivatives was determined by reaction with singlet oxygen acceptors in aqueous and ethanolic solutions; in both solvents ϕ1o2 was enhanced with increasing halogenation.