Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy
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TLDR
Both the theoretical simulation and experimental data show that photobleaching of fluorescein in microscopy is, in general, not a single-exponential process.About:
This article is published in Biophysical Journal.The article was published on 1995-06-01 and is currently open access. It has received 635 citations till now. The article focuses on the topics: Photobleaching & Fluorescence loss in photobleaching.read more
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Bright and Stable Core−Shell Fluorescent Silica Nanoparticles
TL;DR: These nanoparticles are monodisperse in solution, 20 times brighter, and more photostable than their constituent fluorophore, and are amenable to specific labeling of biological macromolecules for bioimaging experiments.
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Array Tomography: A New Tool for Imaging the Molecular Architecture and Ultrastructure of Neural Circuits
TL;DR: A new imaging method, called array tomography, is described, which combines and extends superlative features of modern optical fluorescence and electron microscopy methods and can reveal important but previously unseen features of brain molecular architecture.
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Nanoparticles for bioimaging.
TL;DR: An overview of properties and design of contrast agents such as dye-doped silica nanoparticles, quantum dots and gold nanoparticles for non-invasive bioimaging is provided.
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Long-Lived Emissive Probes for Time-Resolved Photoluminescence Bioimaging and Biosensing
TL;DR: The design and applications of various kinds of long-lived emissive probes for bioimaging and biosensing via time-resolved photoluminescence techniques are summarized and the imaging contrast and sensing sensitivity are remarkably improved.
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Imaging Protein-Protein Interactions Using Fluorescence Resonance Energy Transfer Microscopy
TL;DR: A FRET microscopy method that can be used to determine whether proteins that are colocalized at the level of light microscopy interact with one another, implemented using digital microscopy systems such as a confocal microscope or a wide-field fluorescence microscope coupled to a charge-coupled camera.
References
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Numerical Recipes in C: The Art of Scientific Computing
Mary C. Seiler,Fritz A. Seiler +1 more
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Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.
TL;DR: The theoretical basis and some practical guidelines for simple, rigorous analysis of FPR experiments are presented and some model experiments on aqueous solutions of rhodamine 6G are described.
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Fading of immunofluorescence during microscopy: a study of the phenomenon and its remedy
TL;DR: Study of the kinetics of this photo-bleaching performed by microfluorimetry and on FITC conjugate in solution and the effects of a range of additives incorporated in the mountant suggest that the fading is due to a destructive reaction with protein of the dye in its excited singlet state and that retarding additives suppress this.
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A microfluorimetric study of translational diffusion in erythrocyte membranes.
Reiner Peters,Reiner Peters,Jutta Peters,Jutta Peters,Karl Heinz Tews,Karl Heinz Tews,Wolfgang Bähr,Wolfgang Bähr +7 more
TL;DR: A value of 3 · 10−12 cm2/s was derived from the experimental data as an upper limit for the diffusion coefficient of fluorescein isothiocyanate-labelled compounds in the erythrocyte membrane at 20°–23°C.