Hyun Jin Kim

TL;DR: It is demonstrated that a delivery vehicle composed of gold nanoparticles conjugated to DNA and complexed with cationic endosomal disruptive polymers can deliver Cas9 ribonucleoprotein and donor DNA into a wide variety of cell types and efficiently correct the DNA mutation that causes Duchenne muscular dystrophy in mice via local injection, with minimal off-target DNA damage.

TL;DR: The current status of clinical trials related to siRNA-based cancer therapy is described, as well as the remaining issues that need to be overcome to establish a successful therapy, and various promising design strategies of delivery vehicles for stable and targeted siRNA delivery are introduced.

TL;DR: Polyionic complex micelles that contain the charge-conversional moieties citaconic amide or cis-aconitic amide were developed for cytoplasmic protein delivery and the increase of the charge density on the protein cargo helped the stability of the PIC micells without cross-linking.

TL;DR: It is found that Notch1 activation in GSCs specifically induces expression of the lncRNA, TUG1, which highlights the importance of the Notch-lncRNA axis in regulating self-renewal of glioma cells and provides a strong rationale for targeting Tug1 as a specific and potent therapeutic approach to eliminate the GSC population.

TL;DR: A novel protein-delivery system into cytoplasm based on charge-conversional polyion complex (PIC) micelles is developed, which induces the pH-dependent destabilization of cells.