Isabel Van Audenhove

TL;DR: It is argued that the field of extracellular vesicle (EV) biology needs more transparent reporting to facilitate interpretation and replication of experiments and EV-TRACK, a crowdsourcing knowledgebase that centralizes EV biology and methodology, is described.

TL;DR: Nanobodies are highly adaptable tools for cancer research as they enable specific modulation of targets, enzymatic and non-enzymatic proteins alike and are endowed with considerable therapeutic potential as inhibitors of receptor-ligand pairs and deliverers of drugs or drug-loaded nanoparticles towards tumors.

TL;DR: It is demonstrated that nanobodies enable high resolution protein function mapping in cells and are shown to be important for formation of properly organized invadopodia, MMP‐9 secretion, matrix degradation, and cancer cell invasion.

TL;DR: By means of specific, high-affinity nanobodies inhibiting bundling of fascin or L-plastin, the cooperative mode of action of these bundlers is unraveled and it is shown that the bundlers cannot compensate for each other due to strikingly different bundling characteristics.

TL;DR: As nanobodies can traverse the plasma membrane of eukaryotic cells by means of the enteropathogenic E. coli type III protein secretion system, it is shown that in this promising way of nanobody delivery, actin pedestal formation can be affected following nanobODY injection.