J. Christopher Love

TL;DR: An integrated analytical platform, using arrays of subnanoliter wells (nanowells), for constructing detailed profiles for human B cells comprising the immunophenotypes of these cells, the distribution of isotypes of the secreted antibodies, the specificity and relative affinity for defined antigens, and for a subset of Cells, the genes encoding the heavy and light chains.

TL;DR: The current understanding of a wide range of species is laid out and based on theoretical considerations which are best poised to deliver a step change in cost of manufacturing and volumetric productivity within the next decade are evaluated.

TL;DR: PD-1 expression on CD4 cells identifies a dysfunctional subset refractory to rescue with PD-1 blocking antibodies, suggesting that the influence of immune checkpoint inhibitors may involve recovery of function in the PD—CD4+ T cell compartment.

TL;DR: In this article, an alternative approach that uses arrays of subnanoliter wells coated with recombinant peptide-loaded major histocompatibility complex (MHC) class II monomers to isolate and stimulate individual CD4+ T cells in an antigen-specific manner was described.

TL;DR: This work describes an alternative approach that uses arrays of subnanoliter wells coated with recombinant peptide-loaded MHC class II monomers to isolate and stimulate individual CD4+ T cells in an antigen-specific manner, and should enable direct enumeration of antigen- Specific T cells ex vivo from clinical samples.