Showing papers by "John Douglas Mcpherson published in 1992"
TL;DR: It is shown that the mouse Kv3.1 gene encodes the voltage-gated type l K+ channel in lymphocytes, which is found sparingly in cytotoxic T cells from normal mice and abundantly in a specific T cell subset (CD4- CD8- Thy1+) from mice with autoimmune disease.
93 citations
TL;DR: Expressed HBA currents were voltage-dependent, sodium-selective, and tetrodotoxin-sensitive and, thus, exhibit the biophysical and pharmacological properties characteristic of sodium channels.
Abstract: A cDNA library derived from human cerebral cortex was screened for the presence of sodium channel alpha subunit-specific clones. Ligation of three overlapping clones generated a full-length cDNA clone, HBA, that provided the complete nucleotide sequence coding for a protein of 2005 amino acids. The predicted structure suggests four homologous repeats and exhibits greatest homology and structural similarity to the rat brain sodium channel II. A second cDNA clone, HBB, that encodes a different subtype of sodium channel was isolated. Hybridization of DNA fragments from the 3' untranslated region of HBA and PCR with primers derived from HBB with human-hamster somatic cell hybrids localized these clones to human chromosome 2. In situ hybridization to human metaphase chromosomes mapped the structural genes for both HBA and HBB sodium channels to chromosome 2q23-24.3. The sodium channel HBA gene product was expressed by transfection in CHO cells. Expressed HBA currents were voltage-dependent, sodium-selective, and tetrodotoxin-sensitive and, thus, exhibit the biophysical and pharmacological properties characteristic of sodium channels.
90 citations
TL;DR: The availability of novel genomic clones and the mapping data presented here will make possible the identification of any defect genetically linked to this proteoglycan gene.
57 citations
TL;DR: Evidence implicating glutamatergic synapses in a diversity of physiologic and pathologic processes together with concordance of the chromosomal locales and results of linkage analyses establishes GluR3 and GLUR4 as candidate genes for a number of nervous system disorders including the oculocerebral-renal syndrome of Lowe and a form of manic-depressive illness.
Abstract: The chromosomal localization of human glutamate receptor genes (GluR1- 4) has been established using PCR with DNA isolated from mapping panels of Chinese hamster-human hybrid cell lines and high-resolution fluorescent in situ suppression hybridization. This was accomplished with genomic clones containing putative human homologs of rat GluR 1–4 isolated by high-stringency screening of a cosmid library with the rat cDNAs encoding GluR1–4. The locations of GluR1–4, respectively, are 5q32–33, 4q32–33, Xq25–26, and 11q22–23. Evidence implicating glutamatergic synapses in a diversity of physiologic and pathologic processes together with concordance of the chromosomal locales and results of linkage analyses establishes GluR3 and GluR4 as candidate genes for a number of nervous system disorders including the oculocerebral-renal syndrome of Lowe and a form of manic-depressive illness.
46 citations
TL;DR: Genomic and cDNA clones encoding a novel Shaw-related potassium channel gene have been isolated from mice and humans and localized the gene to human chromosome 19.4 and related gene, human Kv3.4 (KCNC4), respectively.
44 citations
TL;DR: The localization of the D5 dopamine receptor (DRD5) gene to 4p15.1-p 15.33 suggests the possibility that cis-position effects could be responsible for the altered D1-type dopamine receptor number observed in HD tissues or that the DRD5 gene could be a candidate for some of the abnormalities associated with the Wolf-Hirschhorn syndrome.
27 citations
TL;DR: The location of the DDC gene to chromosome 7 is confirmed using a new panel of somatic cell hybrids, and the gene is localized to band p11 on chromosome 7 by fluorescent in situ hybridization.
25 citations
TL;DR: The accomplishments of the Second International Workshop on Human Chromosome 5 as was held May 11--13,1992 at the University of Chicago are described.
Abstract: This report describes the accomplishments of the Second International Workshop on Human Chromosome 5 as was held May 11--13,1992 at the University of Chicago. Included in the report are abstract of individual presentations and a consensus map of the chromosome.
16 citations
TL;DR: Degenerate DNA oligomers coding for highly conserved regions of the voltage-gated calcium channel were synthesized for the polymerase chain reaction (PCR) using DNA from a human brain cDNA library as template to encode part of a protein highly homologous to a subtype of the dihydropyridine-sensitive calcium channel cloned from rabbit heart and rat brain.
13 citations
TL;DR: The polymerase chain reaction technique was used to generate a unique probe complementary to the hydrophobic 5' end of the human cyclophilin B gene that was hybridized to DNAs from human x hamster hybrid somatic cell lines retaining different combinations of human chromosomes.
Abstract: The polymerase chain reaction (PCR) technique was used to generate a unique probe complementary to the hydrophobic 5' end of the human cyclophilin B gene. This unique probe was hybridized to DNAs from human x hamster hybrid somatic cell lines retaining different combinations of human chromosomes. The gene was assigned to chromosome 15.
8 citations