J
John Unitt
Researcher at University of Nottingham
Publications - 37
Citations - 2690
John Unitt is an academic researcher from University of Nottingham. The author has contributed to research in topics: Hit to lead & Transcription factor. The author has an hindex of 17, co-authored 36 publications receiving 2448 citations. Previous affiliations of John Unitt include BioCity Nottingham & Loughborough University.
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Journal ArticleDOI
Oscillations in NF-κB Signaling Control the Dynamics of Gene Expression
David E. Nelson,Adaoha E. C. Ihekwaba,M Elliott,James R. Johnson,C. A. Gibney,B. E. Foreman,Glyn Nelson,Violaine Sée,Caroline A. Horton,David G. Spiller,Steven W. Edwards,H. P. McDowell,John Unitt,Elaine Sullivan,Rachel L. Grimley,Neil Benson,David S. Broomhead,Douglas B. Kell,Michael R. H. White +18 more
TL;DR: Single-cell time-lapse imaging and computational modeling of NF-κB (RelA) localization showed asynchronous oscillations following cell stimulation that decreased in frequency with increased IκBα transcription.
Journal ArticleDOI
Pulsatile Stimulation Determines Timing and Specificity of NF-κB-Dependent Transcription
Louise Ashall,Caroline A. Horton,David E. Nelson,Pawel Paszek,Claire V. Harper,Kate Sillitoe,Sheila Ryan,David G. Spiller,John Unitt,David S. Broomhead,Douglas B. Kell,David A. Rand,Violaine Sée,Michael R. H. White +13 more
TL;DR: Altering the stimulation intervals gave different patterns of NF-κB–dependent gene expression, which supports the idea that oscillation frequency has a functional role in nuclear factor κB regulation.
Journal ArticleDOI
Multi-parameter analysis of the kinetics of NF-kappa B signalling and transcription in single living cells
Glyn Nelson,Luminita Paraoan,David G. Spiller,Geraint J. C. Wilde,Mark Browne,Peter K. Djali,John Unitt,Elaine Sullivan,Eike Floettmann,Michael R. H. White +9 more
TL;DR: The results provide functional evidence that other factors, such as modification of p65 by phosphorylation, or interaction with other proteins such as transcriptional co-activators/co-repressors, may critically modulate the kinetics of transcription through this signalling pathway.
Journal ArticleDOI
Hit-to-lead studies: the discovery of potent, orally active, thiophenecarboxamide IKK-2 inhibitors.
Andrew Baxter,Steve Brough,Anne Cooper,Eike Floettmann,Steve Foster,Christine J Harding,Jason Grant Kettle,Tom McInally,Craig Martin,Michelle Mobbs,Maurice Needham,Pete Newham,Stuart W. Paine,Steve St-Gallay,Sylvia Salter,John Unitt,Yafeng Xue +16 more
TL;DR: A hit-to-lead optimisation programme was carried out on the thiophenecarboxamide high throughput screening hits 1 and 2 resulting in the discovery of the potent and orally bioavailable IKK-2 inhibitor 22.
Journal ArticleDOI
Potent reversible inhibition of myeloperoxidase by aromatic hydroxamates.
Louisa V. Forbes,Tove Sjögren,Françoise Auchère,David W. Jenkins,Bob Thong,David Laughton,Paul Hemsley,Garry Pairaudeau,Rufus Turner,Håkan Eriksson,John Unitt,Anthony J. Kettle +11 more
TL;DR: It is proposed that aromatic hydroxamates bind tightly to the active site of MPO and prevent it from producing hypohalous acids and limiting oxidative stress during inflammation.