J
James R. Johnson
Researcher at University of Liverpool
Publications - 25
Citations - 1767
James R. Johnson is an academic researcher from University of Liverpool. The author has contributed to research in topics: Chemistry & Syntaxin. The author has an hindex of 12, co-authored 23 publications receiving 1580 citations.
Papers
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Journal ArticleDOI
Oscillations in NF-κB Signaling Control the Dynamics of Gene Expression
David E. Nelson,Adaoha E. C. Ihekwaba,M Elliott,James R. Johnson,C. A. Gibney,B. E. Foreman,Glyn Nelson,Violaine Sée,Caroline A. Horton,David G. Spiller,Steven W. Edwards,H. P. McDowell,John Unitt,Elaine Sullivan,Rachel L. Grimley,Neil Benson,David S. Broomhead,Douglas B. Kell,Michael R. H. White +18 more
TL;DR: Single-cell time-lapse imaging and computational modeling of NF-κB (RelA) localization showed asynchronous oscillations following cell stimulation that decreased in frequency with increased IκBα transcription.
Journal ArticleDOI
Building a global alliance of biofoundries
Nathan J. Hillson,Mark X. Caddick,Yizhi Cai,Jose A. Carrasco,Matthew Wook Chang,N Curach,David J. Bell,Rosalind Le Feuvre,Douglas C. Friedman,Xiongfei Fu,Nicholas D. Gold,Markus J. Herrgård,Maciej B. Holowko,Maciej B. Holowko,Maciej B. Holowko,James R. Johnson,Richard A. Johnson,Jay D. Keasling,Richard I. Kitney,Akihiko Kondo,Chenli Liu,Vincent J. J. Martin,Filippo Menolascina,Chiaki Ogino,Nicola J. Patron,Marilene Pavan,Chueh Loo Poh,Isak S. Pretorius,Susan J. Rosser,Nigel S. Scrutton,Marko Storch,Hille Tekotte,Evelyn Travnik,Claudia E. Vickers,Claudia E. Vickers,Wen Shan Yew,Ying-Jin Yuan,Huimin Zhao,Paul S. Freemont +38 more
TL;DR: Biofoundries provide an integrated infrastructure to enable the rapid design, construction, and testing of genetically reprogrammed organisms for biotechnology applications and research.
Journal ArticleDOI
Binding of UNC-18 to the N-terminus of syntaxin is essential for neurotransmission in Caenorhabditis elegans.
TL;DR: Results suggest that binding of UNC-18 to closed syntaxin is dispensable for membrane fusion, whereas interaction with the syntaxin N-terminus is essential for neuronal exocytosis in vivo.
Journal ArticleDOI
Automatic tracking of biological cells and compartments using particle filters and active contours
Hailin Shen,Glyn Nelson,Stephnie Kennedy,David E. Nelson,James R. Johnson,David G. Spiller,Michael R. H. White,Douglas B. Kell +7 more
TL;DR: Prior information from a particle filter provides a reasonable initialisation for the active contour method and prevents it from being trapped in local minima and makes it possible to track complex cellular processes automatically.
Journal ArticleDOI
S-nitrosylation of syntaxin 1 at Cys(145) is a regulatory switch controlling Munc18-1 binding.
Zoë J. Palmer,Rory R. Duncan,James R. Johnson,Lu-Yun Lian,Luciane V. Mello,David Booth,Jeff W. Barclay,Margaret E. Graham,Robert D. Burgoyne,Ian A. Prior,Alan Morgan +10 more
TL;DR: S-nitrosylation of Cys(145) may be a molecular switch to disrupt Munc18-1 binding to the closed conformation of syntaxin 1a, thereby facilitating its engagement with the membrane fusion machinery.