Juan Ortín

TL;DR: The basic biochemistry of NS1 is summarized, in particular its synthesis, structure, and intracellular localization, and the various roles NS1 has in regulating viral replication mechanisms, host innate/adaptive immune responses, and cellular signalling pathways are discussed.

TL;DR: Evidence that cloned (or uncloned) populations of most RNA viruses do not consist of a single genome species of defined sequence, but rather of heterogeneous mixtures of related genomes (quasispecies) is reviewed.

TL;DR: Binding and functional studies with point mutants confirm that the identified site is essential for cap binding in vitro and cap-dependent transcription in vivo by the trimeric polymerase complex, and will allow efficient structure-based design of new anti-influenza compounds inhibiting viral transcription.

TL;DR: It is suggested that H5N1 viruses may require further adaptation to acquire this essential pandemic trait, and the complexity of the genetic basis of influenza virus transmissibility is highlighted.

TL;DR: Inactivation of the NS1 gene, but not the NS2 gene, led to a substantial increase in the splicing efficiency, as shown by the relative accumulations of NS1 and NS2 mRNAs.