K
Kira S. Makarova
Researcher at National Institutes of Health
Publications - 261
Citations - 54191
Kira S. Makarova is an academic researcher from National Institutes of Health. The author has contributed to research in topics: CRISPR & Genome. The author has an hindex of 96, co-authored 235 publications receiving 43747 citations. Previous affiliations of Kira S. Makarova include Rutgers University & San Sebastián University.
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Journal ArticleDOI
CRISPR Arrays Away from cas Genes.
Sergey Shmakov,Irina Utkina,Irina Utkina,Yuri I. Wolf,Kira S. Makarova,Konstantin Severinov,Eugene V. Koonin +6 more
TL;DR: The authors found that up to 25% of the CRISPR arrays in complete bacterial and archaeal genomes are located away from cas genes, indicating that the isolated arrays were either functionally active recently or continue to function.
Journal ArticleDOI
Early vertebrate origin and diversification of small transmembrane regulators of cellular ion transport.
Sergej Pirkmajer,Henriette Kirchner,Leonidas S. Lundell,Pavel V. Zelenin,Juleen R. Zierath,Kira S. Makarova,Yuri I. Wolf,Alexander V. Chibalin +7 more
TL;DR: In this paper, the authors used sequence-based phylogenetic analysis and conservation of local chromosome context to classify small transmembrane proteins such as FXYDs in vertebrates.
Posted ContentDOI
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
Omar O. Abudayyeh,Jonathan S. Gootenberg,Silvana Konermann,Julia Joung,Ian Slaymaker,David Benjamin Turitz Cox,Sergey Shmakov,Kira S. Makarova,Ekaterina Semenova,Leonid Minakhin,Konstantin Severinov,Aviv Regev,Eric S. Lander,Eugene V. Koonin,Feng Zhang +14 more
TL;DR: In vitro biochemical analysis show that C2c2 is guided by a single crRNA and can be programmed to cleave ssRNA targets carrying complementary protospacers and suggest that C 2c2 can be used to develop new RNA-targeting tools.
Journal ArticleDOI
Comparative genomic analysis of evolutionarily conserved but functionally uncharacterized membrane proteins in archaea: Prediction of novel components of secretion, membrane remodeling and glycosylation systems
TL;DR: The currently available collection of archaeal (and bacterial) genomes could be sufficient to identify (almost) all widespread functional modules and develop experimentally testable predictions of their functions.
Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28
Sergey Shmakov,Kira S. Makarova,Eugene V. Koonin,Aaron Smargon,David Benjamin Turitz Cox,Neena Pyzocha,Kaijie Zheng,Ian Slaymaker,Jonathan S. Gootenberg,Omar O. Abudayyeh,Patrick Essletzbichler,Feng Zhang +11 more
TL;DR: Two Class 2 CRISPR-Cas systems (subtype VI-B) that lack Cas1 and Cas2 and encompass a single large effector protein, Cas13b, along with one of two previously uncharacterized associated proteins, Csx27 or CsX28 are established.