K
Kira S. Makarova
Researcher at National Institutes of Health
Publications - 261
Citations - 54191
Kira S. Makarova is an academic researcher from National Institutes of Health. The author has contributed to research in topics: CRISPR & Genome. The author has an hindex of 96, co-authored 235 publications receiving 43747 citations. Previous affiliations of Kira S. Makarova include Rutgers University & San Sebastián University.
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Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems
TL;DR: Evidence is presented that large subunits contained in most of the CRISPR-Cas systems could be homologous to Cas10 proteins which contain a polymerase-like Palm domain and are predicted to be enzymatically active in Type III CRISpr-cas systems but inactivated in Type I systems.
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Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome
Alexis Dufresne,Marcel Salanoubat,Frédéric Partensky,François Artiguenave,Ilka M. Axmann,Valérie Barbe,Simone Duprat,Michael Y. Galperin,Eugene V. Koonin,Florence Le Gall,Kira S. Makarova,Martin Ostrowski,Sophie Oztas,Catherine Robert,Igor B. Rogozin,David J. Scanlan,Nicole Tandeau de Marsac,Jean Weissenbach,Patrick Wincker,Yuri I. Wolf,Wolfgang R. Hess +20 more
TL;DR: The genome of P. marinus SS120 is one of the two smallest genomes of a photosynthetic organism known to date and lacks many genes that are involved in photosynthesis, DNA repair, solute uptake, intermediary metabolism, motility, phototaxis, and other functions that are conserved among other cyanobacteria.
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Classification and evolution of type II CRISPR-Cas systems
TL;DR: Phylogenomic analysis suggests that at least three cas genes, cas1, cas2 and cas4, and theCRISPR repeats of the type II-B system were acquired via recombination with a type I CRISPR-Cas locus, suggesting that type II CRISpr-Cas evolved via recombinations of mobile nuclease genes with type I loci.
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Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28
Aaron Smargon,David Benjamin Turitz Cox,Neena Pyzocha,Kaijie Zheng,Kaijie Zheng,Kaijie Zheng,Ian Slaymaker,Ian Slaymaker,Ian Slaymaker,Jonathan S. Gootenberg,Omar Abudayyeh,Patrick Essletzbichler,Patrick Essletzbichler,Patrick Essletzbichler,Sergey Shmakov,Sergey Shmakov,Kira S. Makarova,Eugene V. Koonin,Feng Zhang +18 more
TL;DR: It is established that two class 2 CRISPR-Cas systems that lack Cas1 and Cas2 and encompass a single large effector protein, Cas13b, along with one of two previously uncharacterized associated proteins, can achieve RNA interference when heterologously expressed.
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Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems
Ines Fonfara,Anaïs Le Rhun,Krzysztof Chylinski,Kira S. Makarova,Anne-Laure Lécrivain,Janek Bzdrenga,Eugene V. Koonin,Emmanuelle Charpentier +7 more
TL;DR: The reported collection of dual-RNA:Cas9 with associated PAMs expands the possibilities for multiplex genome editing and could provide means to improve the specificity of the RNA-programmable Cas9 tool.