Kit Boye

TL;DR: A multiplex PCR with four primer-pairs was designed to identify the five main known SCCmec types and a clear and easily discriminated band pattern was obtained for all five types.

TL;DR: DNA sequence-based typing of the Staphylococcus aureus protein A gene showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureUS DNA samples sent to 10 laboratories.

TL;DR: Evaluated spa typing of methicillin-resistant Staphylococcus aureus isolates from new MRSA patients in Denmark found a 97% agreement between spa types obtained by the two methods, showing that WGS is a reliable method to determine the spa type of MRSA.

TL;DR: It is shown that the rapid global dissemination of a single pathogenic bacterial clone results in local variation in measured recombination rates, and possible explanatory variables include the size and time since emergence of each defined sub-population, variation in transmission dynamics due to host movement, and changes in the bacterial genome affecting the propensity for recombination.

TL;DR: This data indicates that community-onset MRSA with diverse genetic backgrounds is rapidly emerging in this previously low MRSA prevalence area.