A
Ariane Deplano
Researcher at Université libre de Bruxelles
Publications - 123
Citations - 6540
Ariane Deplano is an academic researcher from Université libre de Bruxelles. The author has contributed to research in topics: Methicillin-resistant Staphylococcus aureus & Typing. The author has an hindex of 44, co-authored 120 publications receiving 6075 citations.
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Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains.
Stephen Murchan,Mary E. Kaufmann,Ariane Deplano,Raf De Ryck,Marc Struelens,Christina Elsberg Zinn,Vivian Fussing,Saara Salmenlinna,Jaana Vuopio-Varkila,Névine El Solh,C Cuny,Wolfgang Witte,Panayotis T. Tassios,N. J. Legakis,Willem B. van Leeuwen,Alex van Belkum,A. Vindel,Idoia Laconcha,Javier Garaizar,Saara Haeggman,Barbro Olsson-Liljequist,Ulrika Ransjo,Geoffrey Coombes,Barry Cookson +23 more
TL;DR: This multinational European Union project has established for the first time a European database of representative epidemic MRSA (EMRSA) strains and has compared them by using a new “harmonized” PFGE protocol developed by a consensus approach that has demonstrated sufficient reproducibility to allow the successful comparison of pulsed-field gels between laboratories and the tracking of strains around the EU.
Journal ArticleDOI
Antimicrobial Resistance of Old and Recent Staphylococcus aureus Isolates from Poultry: First Detection of Livestock-Associated Methicillin-Resistant Strain ST398
Mostafa Nemati,Katleen Hermans,Urszula Lipinska,Olivier Denis,Ariane Deplano,Marc Struelens,Luc Devriese,Frank Pasmans,Freddy Haesebrouck +8 more
TL;DR: This is the first report of “livestock-associated” methicillin resistant S. aureus from healthy poultry.
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Epidemiologic typing and delineation of genetic relatedness of methicillin-resistant Staphylococcus aureus by macrorestriction analysis of genomic DNA by using pulsed-field gel electrophoresis.
TL;DR: Genomic DNA macrorestriction analysis was a useful complement to phenotypic methods for delineating epidemic isolates of MRSA, for identifying their nosocomial reservoirs, and for tracing their intra- and interhospital spread.
Journal ArticleDOI
High Interlaboratory Reproducibility of DNA Sequence-Based Typing of Bacteria in a Multicenter Study
Marta Aires-de-Sousa,Kit Boye,H. de Lencastre,Ariane Deplano,Mark C. Enright,Jerome Etienne,Alexander W. Friedrich,Dag Harmsen,A. Holmes,Xander W. Huijsdens,Angela M. Kearns,Alexander Mellmann,Hélène Meugnier,J. K. Rasheed,Emile Spalburg,Birgit Strommenger,Marc Struelens,Fred C. Tenover,Jonathan C. Thomas,Ulrich Vogel,Henrik Westh,Jian-guo Xu,Wolfgang Witte +22 more
TL;DR: DNA sequence-based typing of the Staphylococcus aureus protein A gene showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureUS DNA samples sent to 10 laboratories.
Journal ArticleDOI
Evaluation of Molecular Typing Methods in Characterizing a European Collection of Epidemic Methicillin-Resistant Staphylococcus aureus Strains: the HARMONY Collection
Barry Cookson,D. Ashley Robinson,Alastair B. Monk,Stephen Murchan,Ariane Deplano,Rafael De Ryck,Marc Struelens,Christina Scheel,Vivian Fussing,Saara Salmenlinna,Jaana Vuopio-Varkila,C Cuny,Wolfgang Witte,Panayotis T. Tassios,N. J. Legakis,Willem B. van Leeuwen,Alex van Belkum,A. Vindel,Javier Garaizar,Sara Hæggman,Barbro Olsson-Liljequist,Ulrika Ransjo,Manica Müller-Premru,Waleria Hryniewicz,Angela S. Rossney,Brian O'Connell,Benjamin R. D. Short,Jonathan C. Thomas,Simon J. O’Hanlon,Mark C. Enright +29 more
TL;DR: It is demonstrated that the integration of such technologies is achievable, although common protocols (such as those developed for PFGE) may also be important, as is the use of centralized Internet sites to facilitate data analysis.