L
Leonard Guarente
Researcher at Massachusetts Institute of Technology
Publications - 353
Citations - 86116
Leonard Guarente is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Gene & Saccharomyces cerevisiae. The author has an hindex of 143, co-authored 352 publications receiving 80169 citations. Previous affiliations of Leonard Guarente include Novartis & Harvard University.
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Journal ArticleDOI
Mammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine acetylation.
David B. Lombard,Frederick W. Alt,Hwei Ling Cheng,Jakob Bunkenborg,Ryan S. Streeper,Raul Mostoslavsky,Jennifer Kim,George D. Yancopoulos,David M. Valenzuela,Andrew J. Murphy,Yinhua Yang,Yaohui Chen,Matthew D. Hirschey,Roderick T. Bronson,Marcia C. Haigis,Leonard Guarente,Robert V. Farese,Sherman M. Weissman,Eric Verdin,Bjoern Schwer,Bjoern Schwer +20 more
TL;DR: It is demonstrated that SIRT3 has evolved to control reversible lysine acetylation in this organelle and is shown to be a soluble mitochondrial protein.
Journal ArticleDOI
SIRT4 Inhibits Glutamate Dehydrogenase and Opposes the Effects of Calorie Restriction in Pancreatic β Cells
Marcia C. Haigis,Raul Mostoslavsky,Kevin M. Haigis,Kamau Fahie,Danos C. Christodoulou,Andrew J. Murphy,David M. Valenzuela,George D. Yancopoulos,Margaret Karow,Gil Blander,Cynthia Wolberger,Tomas A. Prolla,Richard Weindruch,Frederick W. Alt,Leonard Guarente +14 more
TL;DR: It is shown that SIRT4 functions in beta cell mitochondria to repress the activity of GDH by ADP-ribosylation, thereby downregulating insulin secretion in response to amino acids, effects that are alleviated during CR.
Journal ArticleDOI
Calorie restriction extends Saccharomyces cerevisiae lifespan by increasing respiration
Su Ju Lin,Matt Kaeberlein,Alex A. Andalis,Lori A. Sturtz,Pierre-Antoine Defossez,Pierre-Antoine Defossez,Valeria C. Culotta,Gerald R. Fink,Leonard Guarente +8 more
TL;DR: In this paper, a model of calorie restriction in yeast Saccharomyces cerevisiae was established and it was shown that the shunting of carbon metabolism toward the mitochondrial tricarboxylic acid cycle and the concomitant increase in respiration play a central part in this process.
Book ChapterDOI
Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast.
TL;DR: This chapter describes plasmids and methods for constructing fusions of any cloned gene to lacZ for study in yeast, which provide powerful tools in the analysis of the expression of yeast genes.
Journal ArticleDOI
Calorie restriction, SIRT1 and metabolism: understanding longevity
Laura Bordone,Leonard Guarente +1 more
TL;DR: Recent findings that are beginning to clarify the mechanisms by which CR results in longevity and robust health, which might open new avenues of therapy for diseases of ageing are summarized.