Michael F. Hirshman

TL;DR: Western blot analysis using the monoclonal antibody mAb 1F8 (specific for the insulin-regulatable glucose transporter) demonstrated increased glucose transporter densities in plasma membranes from insulin-treated hind limb skeletal muscle compared with untreated tissues, while microsomal membranes from the diabetes-treated hamstring skeletal muscle had a concomitant decrease in transporter density.

TL;DR: A dissociation between AS160 protein expression and apparent AS160 PAS phosphorylation among soleus, tibialis anterior, and extensor digitorum longus muscles is discovered and is identified as the AS160 paralog TBC1D1, an obesity candidate gene regulating GLUT4 translocation in adipocytes.

TL;DR: Normal expression of muscle insulin receptors is not needed for the exercise-mediated increase in glucose uptake and glycogen synthase activity in vivo, and the synergistic activation of glucose transport with exercise plus insulin is retained in MIRKO mice, suggesting a phenomenon mediated by nonmuscle cells or by downstream signaling events.

TL;DR: It is demonstrated that treadmill running exercise of rats using two different protocols (intermediate high or high-intensity exhaustive exercise) significantly increases Akt activity and phosphorylation in skeletal muscle composed of various fiber types and that mechanical tension may be a part of the mechanism by which contraction activates Akt in fast-twitch muscles.

TL;DR: It is found that ATP depletion was accelerated in transgenic mice with cardiac-specific overexpression of a dominant negative mutant of the AMPK α2 catalytic subunit during no-flow ischemia, and these hearts developed left ventricular dysfunction manifested by an early and more rapid increase inleft ventricular end-diastolic pressure.