A
Amit Zeisel
Researcher at Karolinska Institutet
Publications - 49
Citations - 13245
Amit Zeisel is an academic researcher from Karolinska Institutet. The author has contributed to research in topics: Cell type & Cellular differentiation. The author has an hindex of 28, co-authored 47 publications receiving 9077 citations. Previous affiliations of Amit Zeisel include Technion – Israel Institute of Technology & Weizmann Institute of Science.
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Journal ArticleDOI
Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq
Amit Zeisel,Ana B. Muñoz-Manchado,Simone Codeluppi,Peter Lönnerberg,Gioele La Manno,Anna Juréus,Sueli Marques,Hermany Munguba,Liqun He,Christer Betsholtz,Christer Betsholtz,Charlotte Rolny,Gonçalo Castelo-Branco,Jens Hjerling-Leffler,Sten Linnarsson +14 more
TL;DR: Large-scale single-cell RNA sequencing is used to classify cells in the mouse somatosensory cortex and hippocampal CA1 region and found 47 molecularly distinct subclasses, comprising all known major cell types in the cortex.
Journal ArticleDOI
RNA velocity of single cells
Gioele La Manno,Gioele La Manno,Ruslan A. Soldatov,Amit Zeisel,Amit Zeisel,Emelie Braun,Emelie Braun,Hannah Hochgerner,Hannah Hochgerner,Viktor Petukhov,Viktor Petukhov,Katja Lidschreiber,Maria Eleni Kastriti,Peter Lönnerberg,Peter Lönnerberg,Alessandro Furlan,Jean Fan,Lars E. Borm,Lars E. Borm,Zehua Liu,David van Bruggen,Jimin Guo,Xiaoling He,Roger A. Barker,Erik Sundström,Gonçalo Castelo-Branco,Patrick Cramer,Patrick Cramer,Igor Adameyko,Sten Linnarsson,Sten Linnarsson,Peter V. Kharchenko +31 more
TL;DR: It is shown that RNA velocity—the time derivative of the gene expression state—can be directly estimated by distinguishing between unspliced and spliced mRNAs in common single-cell RNA sequencing protocols, and expected to greatly aid the analysis of developmental lineages and cellular dynamics, particularly in humans.
Journal ArticleDOI
Molecular Architecture of the Mouse Nervous System.
Amit Zeisel,Hannah Hochgerner,Peter Lönnerberg,Anna Johnsson,Fatima Memic,Job van der Zwan,Martin Häring,Emelie Braun,Lars E. Borm,Gioele La Manno,Simone Codeluppi,Alessandro Furlan,Kawai Lee,Nathan G. Skene,Kenneth D. Harris,Jens Hjerling-Leffler,Ernest Arenas,Patrik Ernfors,Ulrika Marklund,Sten Linnarsson +19 more
TL;DR: RNA sequencing of half a million single cells was used to create a detailed census of cell types in the mouse nervous system and mapped cell types spatially and derived a hierarchical, data-driven taxonomy.
Journal ArticleDOI
Quantitative single-cell RNA-seq with unique molecular identifiers
Saiful Islam,Amit Zeisel,Simon Joost,Gioele La Manno,Pawel Zajac,Maria Kasper,Peter Lönnerberg,Sten Linnarsson +7 more
TL;DR: It is shown that molecular labels—random sequences that label individual molecules—can nearly eliminate amplification noise, and that microfluidic sample preparation and optimized reagents produce a fivefold improvement in mRNA capture efficiency.
Journal ArticleDOI
Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system.
Sueli Marques,Amit Zeisel,Simone Codeluppi,David van Bruggen,Ana Mendanha Falcão,Lin Xiao,Lin Xiao,Huiliang Li,Martin Häring,Hannah Hochgerner,Roman A. Romanov,Roman A. Romanov,Daniel Gyllborg,Ana B. Muñoz-Manchado,Gioele La Manno,Peter Lönnerberg,Elisa M. Floriddia,Fatemah Rezayee,Patrik Ernfors,Ernest Arenas,Jens Hjerling-Leffler,Tibor Harkany,Tibor Harkany,William D. Richardson,Sten Linnarsson,Gonçalo Castelo-Branco +25 more
TL;DR: The dynamics of oligodendrocyte differentiation and maturation are revealed, uncoupling them at a transcriptional level and highlighting oligodendedrocytes heterogeneity in the central nervous system.