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Neville E. Sanjana
Researcher at New York University
Publications - 124
Citations - 21164
Neville E. Sanjana is an academic researcher from New York University. The author has contributed to research in topics: CRISPR & Gene. The author has an hindex of 38, co-authored 109 publications receiving 15921 citations. Previous affiliations of Neville E. Sanjana include McGovern Institute for Brain Research & Massachusetts Institute of Technology.
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Journal ArticleDOI
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
Ophir Shalem,Ophir Shalem,Neville E. Sanjana,Neville E. Sanjana,Ella Hartenian,Xi-Shun Shi,David A. Scott,David A. Scott,Tarjei S. Mikkelsen,Dirk Heckl,Benjamin L. Ebert,David E. Root,John G. Doench,Feng Zhang,Feng Zhang +14 more
TL;DR: This work shows that lentiviral delivery of a genome-scale CRISPR-Cas9 knockout (GeCKO) library targeting 18,080 genes with 64,751 unique guide sequences enables both negative and positive selection screening in human cells, and observes a high level of consistency between independent guide RNAs targeting the same gene and a high rate of hit confirmation.
Journal ArticleDOI
Improved vectors and genome-wide libraries for CRISPR screening.
TL;DR: In this paper, Zhang et al. used a Genome-scale CRISPR Knock-Out (GeCKO) library to identify loss-of-function mutations in a melanoma model.
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High-throughput functional genomics using CRISPR–Cas9
TL;DR: A review of the latest applications of CRISPR-Cas9 in mammalian functional genomics screens is presented in this article, which covers related genome-scale applications of Cas9 for either gene knockout or transcriptional modulation.
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Perturbation of m6A Writers Reveals Two Distinct Classes of mRNA Methylation at Internal and 5′ Sites
Schraga Schwartz,Maxwell R. Mumbach,Marko Jovanovic,Timothy C. Wang,Timothy C. Wang,Karolina Maciag,Karolina Maciag,G. Guy Bushkin,Philipp Mertins,Dmitry Ter-Ovanesyan,Naomi Habib,Davide Cacchiarelli,Davide Cacchiarelli,Neville E. Sanjana,Elizaveta Freinkman,Michael E. Pacold,Michael E. Pacold,Rahul Satija,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,Nir Hacohen,Nir Hacohen,Feng Zhang,Feng Zhang,Feng Zhang,Steven A. Carr,Eric S. Lander,Eric S. Lander,Eric S. Lander,Aviv Regev,Aviv Regev,Aviv Regev +31 more
TL;DR: A dense network of proteins interacting with METTL3, a component of the methyltransferase complex, is identified, and it is shown that three of them (WTAP, METTL14, and KIAA1429) are required for methylation.
Journal ArticleDOI
Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis
Sidi Chen,Sidi Chen,Neville E. Sanjana,Kaijie Zheng,Ophir Shalem,Kyungheon Lee,Xi Shi,David A. Scott,Jun S. Song,Jen Q. Pan,Ralph Weissleder,Hakho Lee,Feng Zhang,Phillip A. Sharp +13 more
TL;DR: In this paper, a genome-wide CRISPR/Cas9-mediated loss-of-function screen in tumor growth and metastasis was described. But the authors focused on the effect of mutations on primary tumor growth positively correlates with the development of metastases.