Showing papers by "Nikolaus J. Sucher published in 2002"
TL;DR: It is shown that recombinantly expressedNR3A in COS cells is biochemically associated with both NR1 and NR2 subunits, and whole cell NMDA-evoked currents are larger in NR3A-deficient mice compared with wild-type mice, and this effect follows a developmental pattern similar to that of NR3B protein expression on Western blots.
Abstract: Recently, we cloned and began to characterize a newN-methyl-d-aspartate receptor (NMDAR) subunit, NR3A. Here we extend our earlier findings by showing that recombinantly expressed NR3A in COS cells...
196 citations
TL;DR: A comprehensive study to delineate the temporal and anatomic expression of NR3A protein in the mammalian brain by using a monoclonal anti‐NR3A antibody is reported, which will facilitate future research on NMDARs by providing clues to possible inclusion of the NR2A subunit in NMD ARs in many brain regions.
Abstract: NR3A is a developmentally regulated N-methyl-D-aspartate receptor (NMDAR) subunit that was previously known as NMDAR-L or chi-1. Unlike other NMDAR subunits, NR3A inhibits the NMDAR-associated ion channel in a novel manner, and a role in synaptogenesis has been suggested for this subunit. Here, we report a comprehensive study to delineate the temporal and anatomic expression of NR3A protein in the mammalian brain by using a monoclonal anti-NR3A antibody. NR3A protein was found to peak at postnatal day (P) 8, and to decrease gradually from P12 to adulthood in the rat central nervous system. Moreover, NR3A protein was heavily expressed in all areas of the isocortex, portions of the amygdaloid nuclei, and selective cell layers and nuclei of the hippocampus, thalamus, hypothalamus, brainstem, and spinal cord. NR3A protein was also expressed in the cerebellar cortex, whereas only weak signal was detected in the previous in situ studies by using riboprobes. At an ultrastructural level, NR3A was associated specifically with asymmetrical synapses and localized to postsynaptic membranes. This information will facilitate future research on NMDARs by providing clues to possible inclusion of the NR3A subunit in NMDARs in many brain regions.
191 citations
TL;DR: A novel method for the fast identification of genetic material utilizing a micro-DNA amplification and analysis device (micro-DAAD) consisting of multiple PCR microreactors with integrated DNA microarrays was developed and successful amplification of the genetic material and the consecutive analysis of the fluorescent-labeled amplicons by the integrated oligonucleotide probes were demonstrated.
Abstract: A novel method for the fast identification of genetic material utilizing a micro-DNA amplification and analysis device (μ-DAAD) consisting of multiple PCR microreactors with integrated DNA microarrays was developed. The device was fabricated in Si-technology and used for the genotyping of Chinese medicinal plants on the basis of differences in the noncoding region of the 5S-rRNA gene. Successful amplification of the genetic material and the consecutive analysis of the fluorescent-labeled amplicons in the μ-DAAD by the integrated oligonucleotide probes were demonstrated. Parallel analysis was performed by loading the four PCR reactors of the μ-DAAD with different samples of 3-μL volume. Temperature sensors and heating elements of the μ-DAAD enable precise temperature control and fast cycling, allowing the rapid completion of a combined amplification and analysis (hybridization) experiment.
107 citations
TL;DR: How TCM provides an extensive and knowledge-rich foundation for implementing a strategically focused pharmacological research program aimed at the development of new drugs is explained.
68 citations
TL;DR: The post-translational fate of N-methyl-D-aspartate receptor (NMDAR) subunit NR1 was characterized in PC12 cells using pulse-chase labeling, block of protein synthesis by cyclohexamide and deglycosylation by endoglycosidase H, indicating the presence of an immature form of NR1 that was retained in the endoplasmic reticulum.
15 citations
07 Aug 2002
TL;DR: In this article, a photolithography process is used to generate flow patterns under an externally applied electric field by electro-osmotic and electrophoretic driving forces to enable fine control of fluid motion in microfluidic devices.
Abstract: A new technology to pattern surface charges, either negatively or positively, using a standard photolithography process is introduced. Unlimited flow patterns can be generated under an externally applied electric field by electro-osmotic and electrophoretic driving forces to enable fine control of fluid motion in microfluidic devices. Two basic flows, shear and vortical, have been realized experimentally to demonstrate the tremendous potential of this technology, especially in analytical microsystems for genomics or cell biology.
8 citations
07 Aug 2002
TL;DR: A simple kinematic relationship was discovered relating the quantity of DNA in each downstream branch to its relative channel cross-sectional area and the concept of equivalent length is introduced to quantify the effect of a bend on the DNA plug motion.
Abstract: The DNA kinetics in micro-capillary electrophoresis is presented. The mobility and diffusion coefficient of 14bp-DNA fragments as a function of concentration in two types of separation sieving matrices, hydroxyethylcellulose (HEC) polymer solution and agarose gel, are extracted through a series of experiments performed in microfabricated devices. In addition, the motion of a DNA plug through a miter bend and splitting a plug in a branch are quantitatively characterized. The concept of equivalent length is introduced to quantify the effect of a bend on the DNA plug motion. In a branching system, a simple kinematic relationship was discovered relating the quantity of DNA in each downstream branch to its relative channel cross-sectional area.
6 citations