Pentao Liu

TL;DR: It is shown that the individual PB insertions can be removed from established iPS cell lines, providing an invaluable tool for discovery, and the traceless removal of reprogramming factors joined with viral 2A sequences delivered by a single transposon from murine iPS lines is demonstrated.

TL;DR: This method is fast, efficient, and reliable and makes it possible to generate cko-targeting vectors in less than 2 wk and should also facilitate the generation of knock-in mutations and transgene constructs, as well as expedite the analysis of regulatory elements and functional domains in or near genes.

TL;DR: It is shown that Wnt3 is expressed before gastrulation in the proximal epiblast of the egg cylinder, then is restricted to the posterior proximalEpiblast and its associated visceral endoderm and subsequently to the primitive streak and mesoderm, and the subsequent establishment of anterior-posterior neural pattern in the ectoderm is dependent on derivatives of the primitive streaks.

TL;DR: It is demonstrated that the Merlin/NF2 tumor suppressor and the YAP oncoprotein function antagonistically to regulate liver development and implicate YAP activation as a mediator of pathologies relevant to Neurofibromatosis 2.

TL;DR: It is reported here that defined deficiencies, inversions and duplications extending to 3-4 cM can be constructed in embryonic stem cells by consecutive targeting of loxP recombination substrates to the end points of a genetic interval followed by Cre-induced recombination, which reconstructs a positive selectable marker which facilitates direct selection of clones with a chromosome structure specific to the relative orientation of theloxP sites.