Steffen Scholpp

TL;DR: AO-LLSM takes high-resolution live-cell imaging of subcellular processes from the confines of the coverslip to the more physiologically relevant 3D environment within whole transparent organisms and creates new opportunities to study the phenotypic diversity of intracellular dynamics, extracellular communication, and collective cell behavior across different cell types, organisms, and developmental stages.

TL;DR: It is shown that Fgf8 morphogen gradients in living zebrafish embryos are established and maintained by two essential factors: fast, free diffusion of single molecules away from the source through extracellular space and a sink function of the receiving cells, regulated by receptor-mediated endocytosis.

TL;DR: The contributions of zebrafish in neural development and adult neurogenesis are summarized.

TL;DR: It is shown that Wnt8a is transported on actin-based filopodia to contact responding cells and activate signalling during neural plate formation in zebrafish, and evidence is provided that such a short-range transport system for Wnt has a long-range signalling function.

TL;DR: It is concluded that proper maturation of the diencephalon requires ZLI-derived Hh signalling, and genetic ablation of the basal plate shows that Hh expression in the ZLI alone is sufficient fordiencephalic differentiation.