Susan M. Janicki

TL;DR: An inducible system to visualize gene expression at the levels of DNA, RNA and protein in living cells is developed, able to correlate changes in chromatin structure with the progression of transcriptional activation allowing for a real-time integrative view of gene expression.

TL;DR: Following energy deprivation, energy-independent motion of mRNPs was observed in a highly ATP-dependent nuclear environment; movements were constrained to chromatin-poor domains and excluded by newly formed chromatin barriers, showing that the energetic requirements of nuclear mRNP trafficking are consistent with a diffusional model.

TL;DR: Using the lac operator/repressor system and two colour variants of green fluorescent protein, a system to visualize a gene and its protein product directly in living cells, allowing us to examine the spatial organization and timing of gene expression in vivo.

TL;DR: It is shown that three classes of PML nuclear bodies can be distinguished, on the basis of their dynamic properties in living cells, and one class is particularly noteworthy in that it moves by a metabolic-energy-dependent mechanism.

TL;DR: The advent of green fluorescent protein technology, its use in photobleaching experiments and the development of methods to rapidly acquire images and analyze complex datasets have opened the door to unraveling the mechanisms of nuclear functions in living cells.