T
Takeshi Kaizuka
Researcher at University of Tokyo
Publications - 17
Citations - 3056
Takeshi Kaizuka is an academic researcher from University of Tokyo. The author has contributed to research in topics: Autophagy & Autophagy-related protein 13. The author has an hindex of 9, co-authored 13 publications receiving 2558 citations. Previous affiliations of Takeshi Kaizuka include Tokyo Medical and Dental University.
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Journal ArticleDOI
Nutrient-dependent mTORC1 Association with the ULK1–Atg13–FIP200 Complex Required for Autophagy
Nao Hosokawa,Taichi Hara,Takeshi Kaizuka,Chieko Kishi,Akito Takamura,Yutaka Miura,Shun-ichiro Iemura,Tohru Natsume,Kenji Takehana,Naoyuki Yamada,Jun-Lin Guan,Noriko Oshiro,Noriko Oshiro,Noboru Mizushima +13 more
TL;DR: A novel mammalian autophagy factor, Atg13, is reported, which forms a stable approximately 3-MDa protein complex with ULK1 and FIP200, and suggests that mTORC1 suppressesAutophagy through direct regulation of the approximately 3,MDa ULK 1-Atg13-FIP200 complex.
Journal ArticleDOI
An Autophagic Flux Probe that Releases an Internal Control
Takeshi Kaizuka,Hideaki Morishita,Yutaro Hama,Satoshi Tsukamoto,Takahide Matsui,Yuichiro Toyota,Akihiko Kodama,Tomoaki Ishihara,Tohru Mizushima,Noboru Mizushima +9 more
TL;DR: The GFP- LC3-RFP-LC3ΔG probe is a simple and quantitative method to evaluate autophagic flux in cultured cells and whole organisms, and re-evaluated previously reported autophagy-modulating compounds, performed a high-throughput screen of an approved drug library, and identified autophile modulators.
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Tti1 and Tel2 Are Critical Factors in Mammalian Target of Rapamycin Complex Assembly
Takeshi Kaizuka,Taichi Hara,Noriko Oshiro,Ushio Kikkawa,Kazuyoshi Yonezawa,Kenji Takehana,Shun-ichiro Iemura,Tohru Natsume,Noboru Mizushima +8 more
TL;DR: This study identified KIAA0406 as a novel mTOR-interacting protein that has sequence homology with Schizosaccharomyces pombe Tti1 and confirmed that Tti 1 binds to Tel2 also in mammalian cells, and Tti2 interacts with and stabilizes all six members of the PIKK family of proteins.
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Dynamic association of the ULK1 complex with omegasomes during autophagy induction.
Eleftherios Karanasios,Eloise Stapleton,Maria Manifava,Takeshi Kaizuka,Takeshi Kaizuka,Noboru Mizushima,Noboru Mizushima,Simon Walker,Nicholas T. Ktistakis +8 more
TL;DR: It is shown that the ULK1 complex colocalises with omegasomes in a PtdIns3P-dependent way, and a complete sequence of steps leading to autophagosome formation is established for the first time.
Journal ArticleDOI
FIP200 regulates targeting of Atg16L1 to the isolation membrane
Taki Nishimura,Taki Nishimura,Takeshi Kaizuka,Ken Cadwell,Ken Cadwell,Mayurbhai Himatbhai Sahani,Tatsuya Saitoh,Shizuo Akira,Herbert W. Virgin,Noboru Mizushima,Noboru Mizushima +10 more
TL;DR: It is found that FIP200, which is involved in proximal events, directly interacts with Atg16L1, one of the downstream Atg factors, in an Atg14‐ and phosphatidylinositol 3‐kinase‐independent manner.